Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody - BSA Free

Western Blot: PDK-1 Antibody [NB100-2384] - Detection of PDK-1 in human heart lysate using NB 100-2384 at 0.5 ug/ml.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Pm, Ze
• Applications: WB, WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody - BSA Free (NB100-2384) is a polyclonal antibody validated for use in WB. Anti-Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an internal portion of the human PDK1 protein sequence (between residues 300-400). [Swiss-Prot Q15118]
• Localization: Mitochondrion
• Predicted Species: Rat (100%), Porcine (91%), Bovine (100%), Zebrafish (91%), Chicken (92%), Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting reported in scientific literature (PMID 28366875)
  • Western Blot 0.5-2 ug/ml
• Application Notes: This PDK1 antibody is useful for Western blot, where a band is seen at ~49 kDa representing PDK1 protein. There are also two higher and one lower non-specific molecular weight bands. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Pyruvate Dehydrogenase Kinase 1/PDK1 Overexpression Lysate
  Human Heart Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  Pyruvate Dehydrogenase Kinase 1/PDK1 Protein (NB100-2384PEP)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25925251).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine and 0.15M NaCl
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.2
• mitochondrial pyruvate dehydrogenase, lipoamide, kinase isoenzyme 1
• PDH kinase 1
• PDK1
• PDK-1
• PKB kinase
• Pyruvate dehydrogenase kinase isoform 1
• pyruvate dehydrogenase kinase, isoenzyme 1
• pyruvate dehydrogenase kinase, isozyme 1
• Pyruvate dehydrogenase lipoamide kinase isozyme 1, mitochondrial

Background

Pyruvate dehydrogenase kinase isoform 1 (PDK1) is a component of the mitochondrial multienzyme complex (PHD) which is responsible for carbohydrate fuel regulation. PDK1 inhbits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.
PDK1 antibodies are useful tools for glucose metabolism research and mitochondrial metabolism studies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Pyruvate Dehydrogenase Kinase 1/PDK1 Antibody (NB100-2384)(4)

Publications using NB100-2384

• Gibier JB, Hemon B, Fanchon M et al. Dual role of MUC1 mucin in kidney ischemia-reperfusion injury: Nephroprotector in early phase, but pro-fibrotic in late phase. Biochim. Biophys. Acta. 2017-03-31 [PMID: 28366875] (IB)
• Pastor-Soler NM, Sutton TA, Mang HE et al. Muc1 is protective during kidney ischemia-reperfusion injury Am J Physiol Renal Physiol. 2015-04-28 [PMID: 25925251] (WB, Mouse)
• Ho N, Coomber BL. Pyruvate dehydrogenase kinase expression and metabolic changes following dichloroacetate exposure in anoxic human colorectal cancer cells. Exp Cell Res 2015-02-01 [PMID: 25536473] (Human)
• Lin HP, Jiang SS, Chuu CP. Caffeic Acid Phenethyl Ester Causes p21 Induction, Akt Signaling Reduction, and Growth Inhibition in PC-3 Human Prostate Cancer Cells. PLoS One. 2012-02-07 [PMID: 22347457]

Bioinformatics

• Gene Symbol: PDK1