Human Pyruvate Dehydrogenase E1 beta subunit - Ready-To-Use ELISA Kit (Colorimetric)

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ELISA: Human Pyruvate Dehydrogenase E1 beta subunit - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40299] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Tissue homogenates, cell lysates, cell culture supernatants and other biological fluids
Standard Curve Range
1.56 - 100 U/L (example only; lot dependent)
Sensitivity
1.2 U/L (example only; lot dependent)

Order Details

Human Pyruvate Dehydrogenase E1 beta subunit - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Standard Curve Range
1.56 - 100 U/L (example only; lot dependent)
Sensitivity
1.2 U/L (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PDHB

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Solution A
  2. Detection Solution B
  3. Instruction manual
  4. Plate sealer for 96 wells
  5. Pre-coated 96T strip plate
  6. Standard Diluent
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human Pyruvate Dehydrogenase E1 beta subunit - Ready-To-Use ELISA Kit (Colorimetric)

  • DKFZp564K0164
  • mitochondrial
  • pyruvate dehydrogenase (lipoamide) beta
  • pyruvate dehydrogenase, E1 beta polypeptide

Background

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PDHB