PU.1/Spi-1 Antibody (2G1)

Western Blot: PU.1/Spi-1 Antibody (2G1) [H00006688-M02] - SPI1 monoclonal antibody (M02), clone 2G1. Analysis of SPI1 expression in Raw 264.7.

Immunocytochemistry/Immunofluorescence: PU.1/Spi-1 Antibody (2G1) [H00006688-M02] - Analysis of monoclonal antibody to SPI1 on HeLa cell. Antibody concentration 10 ug/ml.

ELISA: PU.1/Spi-1 Antibody (2G1) [H00006688-M02] - Detection limit for recombinant GST tagged SPI1 is approximately 0.1ng/ml as a capture antibody.

Product Discontinued

PU.1/Spi-1 Antibody (2G1) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: SPI1 (NP_003111, 1 a.a. ~ 120 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEGFPLVPPPSEDLVPYDTDLYQRQTHEYYPYLSSDGESHSDHYWDFHPHHVHSEFESFAENNFTELQSVQPPQLQQLYRHMELEQMHVLDTPMVPPHPSLGHQVSYLPRMCLQYPSLSP
• Specificity: SPI1 - spleen focus forming virus (SFFV) proviral integration oncogene spi1 (2G1)
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPI1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PU.1/Spi-1 Antibody (2G1)

• hematopoietic transcription factor PU.1
• OF
• PU.1
• SFPI1
• SFPI131 kDa transforming protein
• SPI-1 proto-oncogene
• SPI1
• Spi-1
• SPI-A
• spleen focus forming virus (SFFV) proviral integration oncogene spi1,31 kDa-transforming protein
• transcription factor PU.1

Background

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PU.1/Spi-1 Antibody (H00006688-M02)(1)

Publication using H00006688-M02

• Vegran F, Berger H, Boidot R et al. The transcription factor IRF1 dictates the IL-21-dependent anticancer functions of TH9 cells. Nat. Immunol. 2014-06-29 [PMID: 24973819] (WB, Mouse)

Bioinformatics

• Gene Symbol: SPI1
• Entrez:
  • 6688(Human)
• Uniprot:
  • NP_003111(Human)