PTP alpha/PTPRA Overexpression Lysate (Native)

Images

 
Western Blot: PTP alpha Overexpression Lysate (Adult Normal) [NBL1-14979] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PTP alpha.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PTP alpha/PTPRA Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC222592 and based on accession number NM_002836. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens protein tyrosine phosphatase, receptor type, A (PTPRA), transcript variant 1, mRNA.
Gene
PTPRA

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
88.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PTP alpha/PTPRA

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PTP alpha/PTPRA Overexpression Lysate (Native)

  • EC 3.1.3.48
  • HEPTP
  • HLPR
  • LRP
  • LRPHPTPalpha
  • protein tyrosine phosphatase, receptor type, A
  • Protein-tyrosine phosphatase alpha
  • PTP alpha
  • PTPAreceptor type, alpha polypeptide
  • PTPase-alpha
  • PTPLCA-related phosphatase
  • PTPRA
  • PTPRL2
  • receptor-type tyrosine-protein phosphatase alpha
  • RPTPA
  • R-PTP-alpha
  • tyrosine phosphatase alpha

Background

The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus represents a receptor-type PTP. This PTP has been shown to dephosphorylate and activate Src family tyrosine kinases, and is implicated in the regulation of integrin signaling, cell adhesion and proliferation. Three alternatively spliced variants of this gene, which encode two distinct isoforms, have been reported. [provided by RefSeq]. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for PTP alpha/PTPRA Lysate (NBL1-14979) (0)

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Bioinformatics

Gene Symbol PTPRA
Entrez