PTIP Antibody

Immunohistochemistry-Paraffin: PTIP Antibody [NBP2-33877] - Staining in human testis and liver tissues using anti-PAXIP1 antibody. Corresponding PAXIP1 RNA-seq data are presented for the same tissues.

Western Blot: PTIP Antibody [NBP2-33877] - Analysis in control (vector only transfected HEK293T lysate) and PAXIP1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

Immunocytochemistry/Immunofluorescence: PTIP Antibody [NBP2-33877] - Staining of human cell line U-251 MG shows positivity in vesicles & nucleus but excluded from the nucleoli. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: PTIP Antibody [NBP2-33877] - Staining of human liver shows low expression as expected.

Immunohistochemistry-Paraffin: PTIP Antibody [NBP2-33877] - Staining of human testis shows high expression.

Product Discontinued

PTIP Antibody Summary

• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: SNTVLICKEPTGLKYEKAKEWRIPCVNAQWLGDILLGNFEALRQIQYSRYTAFSLQDPFAPTQHLVLNLLDAWRVPLKVSAELLMSIRLPPKLKQNEVANVQPSSKRARIEDVPPPTKKLTPELTPFVLFTGFEPVQVQQYIKK
• Predicted Species: Rat (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PAXIP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:500 - 1:1000
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for PTIP Antibody

• CAGF28
• PACIP1
• PAX interacting (with transcription-activation domain) protein 1
• PAX transactivation activation domain-interacting protein
• PAX transcription activation domain interacting protein 1 like
• PAX-interacting protein 1
• PAXIP1LCAGF29
• protein encoded by CAG trinucleotide repeats
• PTIPFLJ41049
• TNRC2

Background

PTIP facilitates ATM-mediated activation of p53 and promotes cellular resistance to ionizing radiation. Tandem BRCT (BRCA1 carboxyl-terminal) domains in PTIP and in BRCA1 serve as phosphoserine or phosphothreonine specific binding modules that recognize substrates phosphorylated by the kinases ATM and ATR in response to gamma irradiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PAXIP1
• Uniprot:
  • Q6ZW49(Human)