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PSME4 Antibody [DyLight 650]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 650

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP2-22236AF350
Alexa Fluor 405
NBP2-22236AF405
Alexa Fluor 488
NBP2-22236AF488
Alexa Fluor 532
NBP2-22236AF532
Alexa Fluor 594
NBP2-22236AF594
Alexa Fluor 647
NBP2-22236AF647
Alexa Fluor 700
NBP2-22236AF700
Alexa Fluor 750
NBP2-22236AF750
DyLight 350
NBP2-22236UV
DyLight 405
NBP2-22236V
DyLight 488
NBP2-22236G
DyLight 550
NBP2-22236R
DyLight 594
NBP2-22236DL594
DyLight 680
NBP2-22236FR
DyLight 755
NBP2-22236IR
FITC
NBP2-22236F
HRP
NBP2-22236H
Janelia Fluor 549
NBP2-22236JF549
Janelia Fluor 646
NBP2-22236JF646
Unconjugated
NBP2-22236
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PSME4 Antibody [DyLight 650] Summary

Immunogen
The immunogen this antibody was made to, maps to a region between residue 1 and 50 of human Proteasome (Prosome, Macropain) Activator subunit 4 using the numbering given in entry NP_055429.2 (GeneID 23198).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PSME4
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for PSME4 Antibody [DyLight 650]

  • proteasome (prosome, macropain) activator subunit 4

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Tbp7 Antibody [NBP1-87799] - Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Immunocytochemistry/Immunofluorescence: Tbp7 Antibody [NBP1-87799] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Tbp7 Antibody [NBP1-87799] - Staining of human gall bladder shows nuclear positivity in glandular cells.
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Proteasome 19S 10B
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Western Blot: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Proteasome 19S 10B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Proteasome 19S 10B.
Immunocytochemistry/Immunofluorescence: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Proteasome 19S 10B.
Immunohistochemistry-Paraffin: Proteasome 19S 10B Antibody (1G6) [NBP2-00990] - Staining of paraffin-embedded Human pancreas tissue using anti-Proteasome 19S 10B mouse monoclonal antibody.
Species: Hu, Mu, Rt, Ca, Pm, Pm
Applications: WB, ICC/IF, IHC, IHC-P
PA28 Activator
alpha Subunit/PSME1 A ...
NBP2-75606
Western Blot: PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33) [NBP2-75606] - Analysis of PSME1 on MCF-7 cell (1) and rat lung tissue (2) lysate using anti-PSME1 antibody at 1/500 dilution.
Immunocytochemistry/Immunofluorescence: PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33) [NBP2-75606] - Staining PSME1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: PA28 Activator alpha Subunit/PSME1 Antibody (JU60-33) [NBP2-75606] - Analysis of paraffin-embedded rat kidney tissue using anti-PSME1 antibody. Counter stained with hematoxylin.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
Ribophorin I
Antibody (OTI3H3)
NBP2-01294
Western Blot: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Ribophorin I (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Ribophorin I.
Immunocytochemistry/Immunofluorescence: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - expression of RPN1 on pca cell lines vs normal. Image from verified customer review.
Immunohistochemistry: Ribophorin I Antibody (OTI3H3) [NBP2-01294] - Staining of paraffin-embedded Human pancreas tissue using anti-Ribophorin I mouse monoclonal antibody.
(2 Reviews)
(1 Publication)
Species: Hu, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
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Antibody
NBP1-59322
Western Blot: PTHLH/PTHrP Antibody [NBP1-59322] - HepG2 cell lysate, Antibody Titration: 2.0ug/ml
Immunohistochemistry-Paraffin: PTHLH/PTHrP Antibody [NBP1-59322] - Human Lung Alveolar cells (indicated with arrows), 4-8ug/ml.
Western Blot: PTHLH/PTHrP Antibody [NBP1-59322] - Primary, Antibody Dilution: 1 : 1000 Secondary Antibody: Goat anti-rabbit HRP Secondary, Antibody Dilution: 1 : 2000.
Species: Hu
Applications: WB, IHC, IHC-P
PA28 Activator
gamma Subunit/PSME3 A ...
NBP1-33498
Knockout Validated: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Knockout/KnockdownWild-type (WT) and PSME3 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with PSME3 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Sample (30 ug of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE, antibody diluted at 1:2000.
Immunocytochemistry/Immunofluorescence: PA28 Activator gamma Subunit/PSME3 Antibody [NBP1-33498] - Paraformaldehyde-fixed HeLa, using antibody at 1:500 dilution.
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Ribosomal Protein
S6/RPS6 Antibody
NB100-1595
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Rpn2 Antibody
NBP2-38323
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p70 S6 Kinase/S6K
[p Thr389/412] Antibo ...
NB600-1049
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Immunocytochemistry/Immunofluorescence: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Analysis of Phospho-p70 S6 Kinase in 293 cells.
Immunohistochemistry: p70 S6 Kinase/S6K [p Thr389/412] Antibody [NB600-1049] - Human liver cancer tissue sections
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NBP2-01817
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Western blot shows lysates of human cartilage tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Fibromodulin Monoclonal Antibody (Catalog # MAB5945) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Fibromodulin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22236AF350
Alexa Fluor 405 NBP2-22236AF405
Alexa Fluor 488 NBP2-22236AF488
Alexa Fluor 532 NBP2-22236AF532
Alexa Fluor 594 NBP2-22236AF594
Alexa Fluor 647 NBP2-22236AF647
Alexa Fluor 700 NBP2-22236AF700
Alexa Fluor 750 NBP2-22236AF750
DyLight 350 NBP2-22236UV
DyLight 405 NBP2-22236V
DyLight 488 NBP2-22236G
DyLight 550 NBP2-22236R
DyLight 594 NBP2-22236DL594
DyLight 650 NBP2-22236C
DyLight 680 NBP2-22236FR
DyLight 755 NBP2-22236IR
FITC NBP2-22236F
HRP NBP2-22236H
Janelia Fluor 549 NBP2-22236JF549
Janelia Fluor 646 NBP2-22236JF646
Learn about Custom Antibody Labeling

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Array NBP2-22236C

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Diseases for PSME4 Antibody (NBP2-22236C)

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Bioinformatics

Gene Symbol PSME4