PSMA4 Antibody

Images

 
Western Blot: PSMA4 Antibody [NBP1-52074] - Staining of NIH3T3 (A), Mouse Spleen (B), Rat Spleen (C)) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: PSMA4 Antibody [NBP1-52074] - Staining of K562 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PSMA4 Antibody Summary

Immunogen
Peptide with sequence C-SDANVLTNELR corresponding to internal region according to NP_001096138.1.
Epitope
C-SDANVLTNELR
Specificity
This antibody is expected to recognize both reported isoforms (NP_002780.1; NP_001096138.1). Reported variants represent identical protein: NP_002780.1, NP_001096137.1
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PSMA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Approx. 28 kDa band observed in K562, NIH3T3 lysates and human tonsil, human peripheral blood mononucleocytes and in mouse and rat spleen lysates (calculated MW of 29.5 kDa band according to NP_002780.1).

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Canine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PSMA4 Antibody

  • HC9EC 3.4.25.1
  • HsT17706
  • Macropain subunit C9
  • MGC12467
  • MGC24813
  • Multicatalytic endopeptidase complex subunit C9
  • proteasome (prosome, macropain) subunit, alpha type, 4
  • Proteasome component C9
  • proteasome subunit alpha type-4
  • proteasome subunit HC9
  • Proteasome subunit L
  • PSC9MGC111191

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PSMA4
Entrez
Uniprot