PSMA4 Antibody (2A10-E4)

Images

 
Western Blot: PSMA4 Antibody (2A10-E4) [H00005685-M01] - PSMA4 monoclonal antibody (M01), clone 2A10-E4 Analysis of PSMA4 expression in Hela.
Immunocytochemistry/ Immunofluorescence: PSMA4 Antibody (2A10-E4) [H00005685-M01] - Analysis of monoclonal antibody to PSMA4 on HeLa cell. Antibody concentration 10 ug/ml.
Sandwich ELISA: PSMA4 Antibody (2A10-E4) [H00005685-M01] - Detection limit for recombinant GST tagged PSMA4 is approximately 3ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
2A10-E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

PSMA4 Antibody (2A10-E4) Summary

Immunogen
PSMA4 (AAH05361, 1 a.a. - 261 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKHEEEEAKAEREKKEKEQKEKDK
Specificity
PSMA4 - proteasome (prosome, macropain) subunit, alpha type, 4
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PSMA4
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • ELISA
  • Immunocytochemistry/Immunofluorescence
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.
Reviewed Applications
Read 1 Review rated 5
using
H00005685-M01 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4)
Preservative
No Preservative
Purity
IgG purified

Notes

Quality control test: Antibody Reactive Against Recombinant Protein.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PSMA4 Antibody (2A10-E4)

  • HC9EC 3.4.25.1
  • HsT17706
  • Macropain subunit C9
  • MGC12467
  • MGC24813
  • Multicatalytic endopeptidase complex subunit C9
  • proteasome (prosome, macropain) subunit, alpha type, 4
  • Proteasome component C9
  • proteasome subunit alpha type-4
  • proteasome subunit HC9
  • Proteasome subunit L
  • PSC9MGC111191

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Review for PSMA4 Antibody (H00005685-M01) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using H00005685-M01:
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Images Ratings Applications Species Date Details
Western Blot PSMA4 H00005685-M01
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reviewed by:
Anonymous
WB Human 08/28/2012
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Summary

ApplicationWestern Blot
Sample TestedHeLa lysates
SpeciesHuman

Blocking

Blocking Details5% NFDM in PBST, 1 h RT

Primary Anitbody

Dilution Ratio1:500, O/N at 4C

Secondary Antibody

Secondary Descriptiongoat anti-mouse IgG-HRP
Secondary Concentration1:5000

Details

Detection NotesECL plus

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PSMA4 Antibody (H00005685-M01) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for PSMA4 Antibody (H00005685-M01)

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Diseases for PSMA4 Antibody (H00005685-M01)

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Anonymous
08/28/2012
Application: WB
Species: Human

Bioinformatics

Gene Symbol PSMA4
Entrez
Uniprot