PSMA4 Antibody (2A10-E4) - Azide and BSA Free

Western Blot: PSMA4 Antibody (2A10-E4) [H00005685-M01] - PSMA4 monoclonal antibody (M01), clone 2A10-E4 Analysis of PSMA4 expression in Hela.

Immunocytochemistry/Immunofluorescence: PSMA4 Antibody (2A10-E4) [H00005685-M01] - Analysis of monoclonal antibody to PSMA4 on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: PSMA4 Antibody (2A10-E4) [H00005685-M01] - Detection limit for recombinant GST tagged PSMA4 is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2A10-E4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PSMA4 Antibody (2A10-E4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PSMA4 (AAH05361, 1 a.a. ~ 261 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRRYDSRTTIFSPEGRLYQVEYAMEAIGHAGTCLGILANDGVLLAAERRNIHKLLDEVFFSEKIYKLNEDMACSVAGITSDANVLTNELRLIAQRYLLQYQEPIPCEQLVTALCDIKQAYTQFGGKRPFGVSLLYIGWDKHYGFQLYQSDPSGNYGGWKATCIGNNSAAAVSMLKQDYKEGEMTLKSALALAIKVLNKTMDVSKLSAEKVEIATLTRENGKTVIRVLKQKEVEQLIKKHEEEEAKAEREKKEKEQKEKDK
• Specificity: PSMA4 - proteasome (prosome, macropain) subunit, alpha type, 4
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSMA4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PSMA4 Antibody (2A10-E4) - Azide and BSA Free

• HC9
• HC9EC 3.4.25.1
• HsT17706
• Macropain subunit C9
• MGC12467
• MGC24813
• Multicatalytic endopeptidase complex subunit C9
• proteasome (prosome, macropain) subunit, alpha type, 4
• Proteasome component C9
• proteasome subunit alpha type-4
• proteasome subunit HC9
• Proteasome subunit L
• PSC9
• PSC9MGC111191
• PSMA4

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for PSMA4 Antibody (H00005685-M01) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 08/28/2012

Summary

• Application: Western Blot
• Sample Tested: HeLa lysates
• Species: Human

Bioinformatics

• Gene Symbol: PSMA4
• Entrez:
  • 5685(Human)
• Uniprot:
  • AAH05361(Human)