PSF2 Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

PSF2 Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human GINS2.
  
  

  Source: E. coli

  Amino Acid Sequence: ELVTIIPNFSLDKIYLIGGDLGPFNPGLPVEVPLWLAINLKQRQKCRLLPPEWMDVEKLEKMRDHERKEETFTPMPSPYYMELTKLLLNHASDNIPKADEIRTLVKDMWDTRIAKLRVSADSFVRQQEAHAKLDNLTLMEINTSG

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: GINS2
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-33825.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for PSF2 Recombinant Protein Antigen

• DNA replication complex GINS protein PSF2
• GINS complex subunit 2 (Psf2 homolog)
• GINS complex subunit 2
• HSPC037
• PSF2Pfs2

Background

PSF2, also known as DNA replication complex GINS protein PSF2, is a 185 amino acid protein that is 21 kDa, belongs to the GINS2/PSF2 family, localizes in the nucleus, plays an essential role in the initiation of DNA replication and progression of DNA replication forks, and seems to bind preferentially to single-stranded DNA. This protein is being studied for its involvement in breast cancer. The protein has been also linked to the unwinding of DNA, S Phase, DNA strand elongation, synthesis of DNA, DNA replication, cell cycle, and cell cycle, mitotic pathways where it interacts with CHEK2, GINS4, FANCF, GTF2A1L, KPNA4, LMNB1, and other proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: GINS2