PRPSAP2 Antibody (OTI1E3) - Azide and BSA Free

Western Blot: PRPSAP2 Antibody (OTI1E3) - Azide and BSA Free [NBP2-73676] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRPSAP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRPSAP2.

Immunocytochemistry/Immunofluorescence: PRPSAP2 Antibody (OTI1E3) - Azide and BSA Free [NBP2-73676] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY PRPSAP2 .

Immunohistochemistry: PRPSAP2 Antibody (OTI1E3) - Azide and BSA Free [NBP2-73676] - Staining of paraffin-embedded Human Kidney tissue using anti-PRPSAP2 mouse monoclonal antibody.

Flow Cytometry: PRPSAP2 Antibody (OTI1E3) - Azide and BSA Free [NBP2-73676] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-PRPSAP2 antibody, and then analyzed by flow cytometry.

Immunohistochemistry: PRPSAP2 Antibody (OTI1E3) - Azide and BSA Free [NBP2-73676] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-PRPSAP2 mouse monoclonal antibody.

Immunohistochemistry: PRPSAP2 Antibody (OTI1E3) - Azide and BSA Free [NBP2-73676] - Staining of paraffin-embedded Human liver tissue using anti-PRPSAP2 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1E3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

PRPSAP2 Antibody (OTI1E3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse PRPSAP2 Antibody (OTI1E3) - Azide and BSA Free (NBP2-02893) is a monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human PRPSAP2 (NP_002758) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRPSAP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 40.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for PRPSAP2 Antibody (OTI1E3) - Azide and BSA Free

• MGC117304
• MGC126719
• MGC126721
• PAP4141 kDa phosphoribosypyrophosphate synthetase-associated protein
• phosphoribosyl pyrophosphate synthase-associated protein 2
• phosphoribosyl pyrophosphate synthetase-associated protein 2
• PRPP synthase-associated protein 2

Background

The enzyme phosphoribosylpyrophosphate synthetase (PRS) catalyzes the formation of phosphoribosylpyrophosphate which is a substrate for synthesis of purine and pyrimidine nucleotides, histidine, tryptophan and NAD. PRS exists as a complex with two catalytic subunits and two associated subunits. This gene encodes a non-catalytic associated subunit of PRS. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRPSAP2