PRPF6 Antibody

Western Blot: PRPF6 Antibody [NBP2-99184] - Anti-PRPF6 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 107 kDa. Observed band size: 119 kDa (We are unsure as to the identity of these extra bands).

Immunoprecipitation: PRPF6 Antibody [NBP2-99184] - PRPF6 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg NIH-3T3 Whole Cell Lysate. Lane C: 0.5 mg Hela Whole Cell Lysate4 ul anti-PRPF6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-PRPF6 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 106 kDa. Observed band size: 106 kDa

Product Discontinued

PRPF6 Antibody Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human PRPF6.
• Predicted Species: Human (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRPF6
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for PRPF6 Antibody

• androgen receptor N-terminal domain transactivating protein-1
• ANT-1
• bB152O15.1
• C20orf14
• chromosome 20 open reading frame 14
• hPrp6
• p102 U5 small nuclear ribonucleoprotein particle-binding protein
• pre-mRNA-processing factor 6
• PRP6 homolog
• PRP6 pre-mRNA processing factor 6 homolog (S. cerevisiae)
• PRP6 pre-mRNA processing factor 6 homolog (yeast)
• Prp6
• putative mitochondrial outer membrane protein import receptor
• TOM
• U5 snRNP-associated 102 kDa protein
• U5-102 kDa protein
• U5-102K

Background

PRPF6 is encoded by this gene appears to be involved in pre-mRNA splicing, possibly acting as a bridging factor between U5 and U4/U6 snRNPs in formation of the spliceosome. The encoded protein also can bind androgen receptor, providing a link between transcriptional activation and splicing.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRPF6