Protogenin Antibodies

NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." alt="Protogenin was detected in immersion fixed frozen sections of embryonic mouse neural tube (E9) using 10 µg/mL Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membranes in neuronal cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membranes in neuronal cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> VCTS021). Tissue was stained using the HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." alt="Protogenin was detected in immersion fixed paraffin-embedded sections of human stomach tumor using Sheep Anti-Human Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6795) at 3 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL012) and counterstained with DAPI (blue). Specific staining was localized to nuclei in the neural tube. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_lightbox"> NL012) and counterstained with DAPI (blue). Specific staining was localized to nuclei in the neural tube. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections." class="big_thumb" />

6795-PR) supports the adhesion in the P19 mouse embryonal carcinoma cell line in a dose-dependent manner (orange line), as measured by Calcein AM. Adhesion elicited by Recombinant Human Protogenin (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Protogenin Monoclonal Antibody (Catalog # MAB6795). The ND₅₀ is typically 0.4-2.4 µg/mL." class="big_lightbox"> 6795-PR) supports the adhesion in the P19 mouse embryonal carcinoma cell line in a dose-dependent manner (orange line), as measured by Calcein AM. Adhesion elicited by Recombinant Human Protogenin (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Protogenin Monoclonal Antibody (Catalog # MAB6795). The ND₅₀ is typically 0.4-2.4 µg/mL." alt="Recombinant Human Protogenin (Catalog # 6795-PR) supports the adhesion in the P19 mouse embryonal carcinoma cell line in a dose-dependent manner (orange line), as measured by Calcein AM. Adhesion elicited by Recombinant Human Protogenin (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Protogenin Monoclonal Antibody (Catalog # MAB6795). The ND₅₀ is typically 0.4-2.4 µg/mL." class="big_thumb" />