Protogenin Antibody (814403) [Unconjugated]

Recombinant Human Protogenin (Catalog # 6795-PR) supports the adhesion in the P19 mouse embryonal carcinoma cell line in a dose-dependent manner (orange line), as measured by Calcein AM. Adhesion elicited by Recombinant Human Protogenin (10 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Protogenin Monoclonal Antibody (Catalog # MAB6795). The ND50 is typically0.4-2.4 µg/mL.

• Reactivity: Hu
• Applications: B/N
• Clone: 814403
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Protogenin Antibody (814403) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Protogenin
  Phe36-Ala952
  Accession # Q2VWP7
• Specificity: Detects human Protogenin in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRTG
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.4-2.4 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protogenin Antibody (814403) [Unconjugated]

• FLJ12795
• FLJ13221
• FLJ25756
• IGDCC5
• immunoglobulin superfamily, DCC subclass, member 5
• Protein Shen-Dan
• protogenin homolog (Gallus gallus)
• Protogenin
• PRTG
• RPTG
• Shen-Dan

Background

PRTG (Protogenin; also Protein Shen-Dan) is a 175-180 kDa transmembrane (TM) member of the DEAL (DCC et al) family of proteins. It is expressed in the embryo in the period before E10.5 in mouse (day 28 in human), and appears in a widespread distribution. Through homophilic intercellular interaction, it regulates initial epiblast cell migration/ingression, thus impacting somite organization. Via its actions as a neural tube epithelial cell receptor, it binds soluble 60 kDa ERdj3, resulting in a suppression of neuron differentiation. Mature human PRTG is an 1115 amino acid (aa) type I TM glycoprotein. It contains a 917 aa extracellular domain (ECD)
(aa 36-952) and a 180 aa cytoplasmic region. The ECD possesses four consecutive Ig-like domains (aa 36-411) followed by five FN type III domains (aa 419-914). There is one potential splice variant that shows a 49 aa substitution for Ala325 coupled to a deletion of aa 516-541. The ECD of human PRTG shares 93% aa identity with the ECD of mouse PRTG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRTG
• Uniprot:
  • Q2VWP7(Human)