Protein O-Glucosyltransferase 1/POGLUT1/KTELC1 Antibody (737509)

Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Protein O-Glucosyltransferase 1/POGLUT Monoclonal Antibody (Catalog # MAB6437) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Protein O-Glucosyltransferase 1/POGLUT at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 737509
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Protein O-Glucosyltransferase 1/POGLUT1/KTELC1 Antibody (737509) Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Protein O-Glucosyltransferase 1/POGLUT1
  Arg24-Leu388
  Accession # Q8NBL1
• Specificity: Detects human Protein O-Glucosyltransferase 1/POGLUT1 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: POGLUT1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Protein O-Glucosyltransferase 1/POGLUT1/KTELC1 Antibody (737509)

• 9630046K23Rik
• C3orf9
• CAP10-like protein, 46 kDa
• chromosome 3 open reading frame 9
• CLP46
• EC 2.4.1.-
• hCLP46CAP10-like 46 kDa protein
• KDELC family like 1
• KDELCL1
• KTEL (Lys-Tyr-Glu-Leu) containing 1
• KTEL motif-containing protein 1
• KTELC1
• MDS010
• MDSRP
• MDSRPKTELC1
• MGC32995
• Myelodysplastic syndromes relative protein
• POGLUT1
• protein O-glucosyltransferase 1
• Rumi
• x 010 protein

Background

O-Glucosyltransferase1 (POGLUT1) is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase, that adds glucose to serine residues within the consensus sequence of C1‑X‑S‑X‑P‑C2 in Notch EGF repeats, thereby regulating cell-fate decisions (1). It is also known as CAP10-like protein (2) and KTELC1 due to the highly conserved CAP10 domain and the presence of an ER-retaining motif, KTEL, at the C‑terminus. The human gene is reported to be involved in the pathogenesis of both acute myelogenous and T‑acute lymphoblastic leukemias (3). POGLUT1 has hydrolase activity on UDP-Glc in a phosphatase-coupled glycosyltransferase assay (4).

1. Acar, M. et al. (2008) Cell 132:247.
2. Teng, Y. et al. (2006) Gene 371:7
3. Wang, Y. et al. (2010) Genet Test Mol Biomarkers 14:127.
4. Wu, Z.L. et al. (2010) Glycobiology, in press.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POGLUT1
• Uniprot:
  • Q8NBL1()