Protein kinase-like protein SgK493 Overexpression Lysate (Native)

Images

 
Western Blot: Protein kinase-like protein SgK493 Overexpression Lysate (Adult Normal) [NBP2-05664] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Protein ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Protein kinase-like protein SgK493 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_612379. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens Protein kinase-like protein SgK493, mRNA.
Gene
PKDCC

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
33.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Protein kinase-like protein SgK493

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Protein kinase-like protein SgK493 Overexpression Lysate (Native)

  • EC 2.7
  • FLJ18197
  • MGC125960
  • protein kinase domain containing, cytoplasmic homolog (mouse)
  • protein kinase domain-containing protein, cytoplasmic
  • Protein kinase-like protein SgK493
  • SgK493
  • Sugen kinase 493
  • Vertebrate lonesome kinase
  • Vlk

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-30052
Species: Hu, Mu, Rt, Av, Ch, GP, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IHC-WhMt
H00256076-P01
Species: Hu
NBP2-57614
Species: Hu
Applications: ICC/IF
NB100-56077
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP1-90367
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF6144
Species: Hu
Applications: WB, Simple Western
NBP2-13306
Species: Hu
Applications: WB, IHC, IHC-P
AF-242-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready
NBP2-57299
Species: Hu
Applications: WB, ICC/IF, IHC-P
NBP1-54467
Species: Hu, Mu, Rt, Ch, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P, IP
NBP2-24464
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC-P
AF3075
Species: Hu
Applications: WB, ICC
NBP2-61420
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC-P
NB100-56630
Species: Hu
Applications: WB
NBP2-05664
Species: Hu
Applications: WB

Publications for Protein kinase-like protein SgK493 Lysate (NBP2-05664) (0)

There are no publications for Protein kinase-like protein SgK493 Lysate (NBP2-05664).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Protein kinase-like protein SgK493 Lysate (NBP2-05664) (0)

There are no reviews for Protein kinase-like protein SgK493 Lysate (NBP2-05664). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Protein kinase-like protein SgK493 Lysate (NBP2-05664) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Protein kinase-like protein SgK493 Products

Bioinformatics Tool for Protein kinase-like protein SgK493 Lysate (NBP2-05664)

Discover related pathways, diseases and genes to Protein kinase-like protein SgK493 Lysate (NBP2-05664). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Protein kinase-like protein SgK493 Lysate (NBP2-05664)

Discover more about diseases related to Protein kinase-like protein SgK493 Lysate (NBP2-05664).
 

Pathways for Protein kinase-like protein SgK493 Lysate (NBP2-05664)

View related products by pathway.

Research Areas for Protein kinase-like protein SgK493 Lysate (NBP2-05664)

Find related products by research area.

Blogs on Protein kinase-like protein SgK493

There are no specific blogs for Protein kinase-like protein SgK493, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Protein kinase-like protein SgK493 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol PKDCC