Protein Kinase D2 [p Ser876] Antibody

Western Blot: Protein Kinase D2 [p Ser876] Antibody [NBP2-29673] - Unstimlated (lane 1) and PDBu stimulated (lane 2) HEK293 cell lysates were probed with anti-phospho-PKD2 (Ser876) (2ug/ml).

Product Discontinued

Protein Kinase D2 [p Ser876] Antibody Summary

• Immunogen: synthetic peptide corresponding to amino acids surrounding phosphorylated Serine 876 of human PKD2.
• Modification: p Ser876
• Specificity: phospho-PKD2 (Ser876),.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRKD2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:100-1:2000
• Theoretical MW: 105 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris-Glycine (phH 7.4), 0.15M NaCl and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

Relevant Entrez Gene Numbers: NM_016457

Alternate Names for Protein Kinase D2 [p Ser876] Antibody

• DKFZP586E0820
• EC 2.7.11
• EC 2.7.11.13
• nPKC-D2
• PKD2HSPC187
• PRKD2
• protein kinase D2
• serine/threonine-protein kinase D2

Background

Protein kinases constitute a large superfamily of enzymes with key regulatory functions in nearly all signal transmission processes of eukaryotic cells; PKD2 is a novel phorbol ester and growth factor-stimulated protein kinase (1). PKD2 is widely expressed in human and murine tissues and in cell lines such as HL60. In vivo, phorbol ester has been shown to autophosphorylate PKD2 in a synergistic fashion. Phosphorylation of Ser(876) of PKD2 correlated with the activation status of the kinase. The first zinc finger-like domain shares 88% identity to the corresponding domains in PKD/PKC-mu and PKC-nu (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRKD2
• Uniprot:
  • Q9BZL6(Human)