Protein Kinase D2 Antibody (2E4)

Images

 
Western Blot: Protein Kinase D2 Antibody (2E4) [H00025865-M01] - PRKD2 monoclonal antibody (M01), clone 2E4 Analysis of PRKD2 expression in HeLa.
Immunohistochemistry-Paraffin: Protein Kinase D2 Antibody (2E4) [H00025865-M01] - Analysis of monoclonal antibody to PRKD2 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 1 ug/ml.
Sandwich ELISA: Protein Kinase D2 Antibody (2E4) [H00025865-M01] - Detection limit for recombinant GST tagged PRKD2 is approximately 1ng/ml as a capture antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clone
2E4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Protein Kinase D2 Antibody (2E4) Summary

Immunogen
PRKD2 (-, 1 a.a. ~ 110 a.a) partial recombinant protein with GST tag.MDESEDSGVIPGSHSENALHASEEEEGEGGKAQSSLGYIPLMRVVQSVRHTTRKSSTTLREGWVVHYSNKDTLRKRHYWRLDCKCITLFQNNTTNRYYKEIPLSEILTVE
Specificity
PRKD2 - protein kinase D2
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PRKD2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry-paraffin and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Protein Kinase D2 Antibody (2E4)

  • DKFZP586E0820
  • EC 2.7.11
  • EC 2.7.11.13
  • nPKC-D2
  • PKD2
  • PKD2HSPC187
  • PRKD2
  • protein kinase D2
  • serine/threonine-protein kinase D2

Background

The protein encoded by this gene belongs to the protein kinase D (PKD) family of serine/threonine protein kinases. This kinase can be activated by phorbol esters as well as by gastrin via the cholecystokinin B receptor (CCKBR) in gastric cancer cells. It can bind to diacylglycerol (DAG) in the trans-Golgi network (TGN) and may regulate basolateral membrane protein exit from TGN. Alternative splicing results in multiple transcript variants encoding different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRKD2
Entrez
OMIM
Uniprot