Proteasome subunit beta type 4 Antibody (6G7-E8)

Immunohistochemistry-Paraffin: Proteasome subunit beta type 4 Antibody (6G7-E8) [H00005692-M01] - Analysis of monoclonal antibody to PSMB4 on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

Sandwich ELISA: Proteasome subunit beta type 4 Antibody (6G7-E8) [H00005692-M01] - Detection limit for recombinant GST tagged PSMB4 is approximately 0.3ng/ml as a capture antibody.

Product Discontinued

Proteasome subunit beta type 4 Antibody (6G7-E8) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PSMB4 (AAH00331, 1 a.a. ~ 264 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEAFLGSRSGLWAGGPAPGQFYRIPSTPDSFMDPASALYRGPITRTQNPMVTGTSVLGVKFEGGVVIAADMLGSYGSLARFRNISRIMRVNNSTMLGASGDYADFQYLKQVLGQMVIDEELLGDGHSYSPRAIHSWLTRAMYSRRSKMNPLWNTMVIGGYADGESFLGYVDMLGVAYEAPSLATGYGAYLAQPLLREVLEKQPVLSQTEARDLVERCMRVLYYRDARSYNRFQTATVTEKGVEIEGPLSTETNWDIAHMISGFE
• Specificity: PSMB4 - proteasome (prosome, macropain) subunit, beta type, 4
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PSMB4
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Proteasome subunit beta type 4 Antibody (6G7-E8)

• EC 3.4.25.1
• HN3
• HsBPROS26
• HsN3
• Macropain beta chain
• Multicatalytic endopeptidase complex beta chain
• PROS26
• PROS-26
• PROS26PROS-26
• proteasome (prosome, macropain) subunit, beta type, 4
• Proteasome beta chain
• Proteasome chain 3
• Proteasome subunit beta type 4
• proteasome subunit beta type-4
• proteasome subunit HsN3
• proteasome subunit, beta type, 4,26 kDa prosomal protein
• PSMB4

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Proteasome subunit beta type 4 Antibody (H00005692-M01)(1)

Publication using H00005692-M01

• Zapatero Almudena, Morente Manuel, Nieto Santiago et al. Predictive value of PAK6 and PSMB4 expression in patients with localized prostate cancer treated with dose-escalation radiation therapy and androgen deprivation therapy. Urol Oncol. 2014-06-16 [PMID: 24946957]

Bioinformatics

• Gene Symbol: PSMB4
• Entrez:
  • 5692(Human)
• OMIM:
  • 602177(Human)