Proteasome 20S beta 3 Antibody

Western Blot: Proteasome 20S beta 3 Antibody [NBP1-52079] - (1ug/ml) staining of K562 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Proteasome 20S beta 3 Antibody Summary

• Immunogen: Peptide with sequence C-NLYELKEGRQ corresponding to internal region according to NP_002786.2.
• Epitope: C-NLYELKEGRQ
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PSMB3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 24 kDa band observed in K562 lysates (calculated MW of 22.9 kDa band according to NP_002786.2).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Proteasome 20S beta 3 Antibody

• EC 3.4.25.1
• HC10-II
• MGC4147
• proteasome (prosome, macropain) subunit, beta type, 3
• Proteasome 20S beta 3
• Proteasome chain 13
• Proteasome component C10-II
• proteasome subunit beta type-3
• Proteasome theta chain
• PSMB3

Background

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Pseudogenes have been identified on chromosomes 2 and 12.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Proteasome 20S beta 3 Antibody (NBP1-52079)(1)

Publication using NBP1-52079

• Ewing RM, Chu P, Elisma F et al. Large-scale mapping of human protein-protein interactions by mass spectrometry. Mol Syst Bio 2007-01-01 [PMID: 17353931]

Bioinformatics

• Gene Symbol: PSMB3
• Entrez:
  • 5691(Human)
  • 26446(Mouse)
  • 29676(Rat)
• Uniprot:
  • NP_002786.2(Human)