Protease Inhibitor 15 Antibody

Western Blot: Protease Inhibitor 15 Antibody [NBP2-11581] - HeLa lysate (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Protease Inhibitor 15 Antibody [NBP2-11581] - Human Skeletal Muscle. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Protease Inhibitor 15 Antibody Summary

• Immunogen: Peptide with sequence C-RTGRYRSILQLVKP corresponding to internal region according to NP_056970.1.
• Epitope: CRTGRYRSILQLVKP
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PI15
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/mL
  • Peptide ELISA Detection limit 1:1000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 33 kDa band observed in HeLa lysates (calculated MW of 29.1 kDa band according to NP_056970.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Protease Inhibitor 15 Antibody

• 25 kDa trypsin inhibitor
• CRISP8
• CRISP-8
• Cysteine-rich secretory protein 8
• P24TI
• p25TI
• P25TIDKFZp686F0366
• peptidase inhibitor 15
• PI-15
• protease inhibitor 15
• sugarCrisp

Background

Protease Inhibitor 15 encodes a trypsin inhibitor. The protein shares similarity to insect venom allergens, mammalian testis-specific proteins and plant pathogenesis-related proteins. It is frequently expressed in human neuroblastoma and glioblastoma cell lines, and thus may play a role in the central nervous system.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Protease Inhibitor 15 Antibody (NBP2-11581)(1)

Publication using NBP2-11581

• Kaplan F, Ledoux P, Kassamali FQ et al. A novel developmentally regulated gene in lung mesenchyme: homology to a tumor-derived trypsin inhibitor. Am J Physiol 1999-06-01 [PMID: 10362728]

Bioinformatics

• Gene Symbol: PI15
• Entrez:
  • 51050(Human)
  • 94227(Mouse)
  • 301489(Rat)