Prostaglandin I2 Synthase Antibody (852307) [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB
• Clone: 852307
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Prostaglandin I2 Synthase Antibody (852307) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Prostaglandin I2 Synthase
  Arg21-Pro500
  Accession # Q16647
• Specificity: Detects human Prostaglandin I2 Synthase in ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Prostaglandin I2 Synthase Antibody (852307) [Alexa Fluor® 488]

• CYP8
• CYP8A1
• CYP8A1PTGI
• CYP8prostacyclin synthase
• EC 5.3.99.4
• MGC126858
• MGC126860
• PGIS
• PGIScytochrome P450, family 8, subfamily A, polypeptide 1
• Prostacyclin Synthase
• prostaglandin I2 (prostacyclin) synthase
• Prostaglandin I2 synthase
• PTGI
• PTGIS

Background

PTGIS (Prostacyclin synthase; also CYP8A1, PGIS and Prostaglandin I2 synthase) is a 50-52 kDa (57 kDa predicted) microsomal, integral membrane member of Family 8 in the cytochrome P450 superfamily of molecules. It is expressed by skeletal and smooth muscle, fibroblasts, macrophages and endothelium. Prostaglandin I2/prostacyclin and thromboxane A2/TXA2 are two lipid mediators that are derived from the same precursor (PGH2). Acting upon PGH2, TXA2 is created through the action of TX synthase, while PGI2 is generated through the action of PTGIS/PGIS. Although they share a common precursor, they exhibit opposite effects on the vasculature, with PGI2 reported to block platelet aggregation, induce vasodilation, and inhibit smooth muscle proliferation. It apparently does so by binding to either the IP receptor or PPAR gamma . Human PTGIS is 500 amino acids (aa) in length. It is a single span transmembrane protein that contains an N-terminal transmembrane segment (aa 1-20) coupled to a lengthy 480 aa cytoplasmic region. Over aa 21-500, human PTGIS shares 86% aa sequence identity with mouse PTGIS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.