Prohibitin 2 Antibody

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Western Blot: Prohibitin 2 Antibody [NB100-1809] - Detection of Human and Mouse REA on HeLa whole cell lysate using NB100-1809. REA was also immunoprecipitated using rabbit anti-REA antibodies NB100-1807 and NB100-1808.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
 

Independent Antibodies

 

Biological Strategies

     

Order Details

Prohibitin 2 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 250 and the C-terminus (residue 299) of human Repressor of Estrogen Activity (Prohibitin 2) using the numbering given in entry NP_009204.1 (GeneID 11331).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PHB2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:10000
  • Immunoprecipitation 1-4 ug/mg of lysate
Positive Control
Prohibitin 2 Lysate (NBL1-14349)
Publications
Read Publications using
NB100-1809 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Prohibitin 2 Antibody

  • BAP
  • Bap37
  • BCAP37
  • B-cell associated protein
  • D-Prohibitin
  • MGC117268
  • p22
  • PHB2
  • PNAS-141
  • Prohibitin 2
  • prohibitin-2
  • REA
  • REAB-cell receptor-associated protein BAP37
  • Repressor of estrogen receptor activity

Background

REA (repressor of estrogen receptor activity), also known as prohibitin 2, has been shown to exhibit a variety of functions that includes mitochondrial chaperone activity, cell cycle regulation, and transcriptional regulation. REA localizes to the mitochondria and associates with mitochondrial complex I where it may play a role in mitochondrial metabolism. REA interacts with pRb to inhibit E2F-mediated transcription as well as the estrogen receptor to repress estrogen-dependent transcription via recruitment of histone deacetylases. Alternate names for REA include B-cell receptor-associated protein BAP37, D-prohibitin, PHB2, BAP, p22, BCAP37, PNAS-141, and MGC117268.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Prohibitin 2 Antibody (NB100-1809)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


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IP
(1)
WB
(1)
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Filter By Species
Human
(1)
All Species

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PHB2
Entrez
Uniprot