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Proenkephalin Antibody

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Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963] - Staining of PFA-perfused cryosection of Rat Striatum. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik Hrabovszky, Inst, Exp, Med., Budapest, Hungary.
Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963] - Staining of PFA-perfused cryosection of Rat Striatum. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by Prof. Erik ...read more
Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-20963] - Staining of PFA-perfused cryosection of Mouse Stria terminalis. HRP-staining with Ni-DAB after Biotin-SP-anti-goat amplification. Data obtained by ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications IHC, IHC-Fr, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

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Proenkephalin Antibody Summary

Immunogen
Peptide with sequence C-YKDSSKQDESH corresponding to internal region according to Uniprot Mouse NP_001002927.1.
Epitope
C terminus
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
PENK
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 0.02 - 0.05 ug/ml
  • Immunohistochemistry-Frozen 0.02-0.05 ug/ml
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 40 kDa band in rat brain lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8 kDa band according to mouse NP_001002927.1 and 30.9 kDa band according to rat NP_058835.1. The 40 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-Fr: Rat brain (striatum) shows staining of dense enkephalinergic axon plexus. Mouse brain (stria terminalis) shows staining of a dense enkephalinergic fibre network in the bed nucleus.
Publications
Read Publication using NBP1-20963.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for Proenkephalin Antibody

  • enkephalin A
  • preproenkephalin
  • proenkephalin
  • proenkephalin-A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: c-Fos Antibody (2H2) [NBP2-50037] - Analysis of cell lysates using mouse c-Fos mAb, dilution 1:1,000 (Green), and rabbit GAPDH pAb, dilution 1:20,000 (Red) used as a loading control. [1] protein standard (red), [2] HeLa cells in serum free media. [3] HeLa cells stimulated with 20% fetal bovine serum for 2hrs after 36hrs in serum free media. [4] rat cortical neurons. [5] rat cortical neurons treated with membrane depolarization buffer for 5hrs. Multiple bands at 50-65kDa in stimulated or treated cell lysates correspond to different forms of the c-Fos proten. The single band at 37 kDa represents GAPDH protein.
Immunocytochemistry/Immunofluorescence: c-Fos Antibody (2H2) [NBP2-50037] - Section of rat hippocampus stained with mouse monoclonal antibody to c-FOS NBP2-50037 in red and counterstained with rabbit polyclonal antibody to FOX3/NeuN. DAPI reveals nuclei of neurons and glia in blue. The hippocampal neurons stain green for FOX3/NeuN and a few also are expressing c-FOS, and so appear orange. These cells were spontaneously active at the time the animal was sacrificed.
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Immunocytochemistry/Immunofluorescence: Substance P Antibody [NB300-187] - Staining of trigeminal ganglia from P3-P5 rat pups. BMC Neuroscience 2010, 11:32doi:10.1186/1471-2202-11-32
Immunohistochemistry: Substance P Antibody [NB300-187] - Spinal Cord Dorsal Horn (Human).
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Publications for Proenkephalin Antibody (NBP1-20963)(1)


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Bioinformatics

Gene Symbol PENK
Entrez
Uniprot