Prodynorphin/PDYN Antibody - BSA Free

Immunohistochemistry: Prodynorphin/PDYN Antibody [NBP3-38195] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using Prodynorphin/PDYN Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry: Prodynorphin/PDYN Antibody [NBP3-38195] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using Prodynorphin/PDYN Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Western Blot: Prodynorphin/PDYN Antibody [NBP3-38195] - Western blot analysis of various lysates using Prodynorphin/PDYN Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

Immunohistochemistry: Prodynorphin/PDYN Antibody [NBP3-38195] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using Prodynorphin/PDYN Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry: Prodynorphin/PDYN Antibody [NBP3-38195] - Immunohistochemistry analysis of paraffin-embedded Rat brain using Prodynorphin/PDYN Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Prodynorphin/PDYN Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Prodynorphin/PDYN Antibody - BSA Free (NBP3-38195) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 21-254 of human Prodynorphin/PDYN (NP_077722.1).
  
  Sequence:
  DCLSRCSLCAVKTQDGPKPINPLICSLQCQAALLPSEEWERCQSFLSFFTPSTLGLNDKEDLGSKSVGEGPYSELAKLSGSFLKELEKSKFLPSISTKENTLSKSLEEKLRGLSDGFREGAESELMRDAQLNDGAMETGTLYLAEEDPKEQVKRYGGFLRKYPKRSSEVAGEGDGDSMGHEDLYKRYGGFLRRIRPKLKWDNQKRYGGFLRRQFKVVTRSQEDPNAYSGELFDA
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PDYN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Prodynorphin/PDYN Antibody - BSA Free

• ADCA
• Beta-neoendorphin-dynorphin
• leu-enkephalin
• leumorphin
• neoendorphin-dynorphin-enkephalin prepropeptide
• PENKB
• preprodynorphin
• preproenkephalin B
• proenkephalin-B
• rimorphin
• SCA23

Background

The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PDYN