Proenkephalin Antibody

Western Blot: Proenkephalin Antibody [NBP1-06066] - (0.3ug/ml) staining of Human Adrenal Gland lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Frozen: Proenkephalin Antibody [NBP1-06066] - Section of Human Hypothalamus shows staining of dense enkephalinergic axon plexus and scattered neuronal cell bodies (higher magnification inset) in the human infundibular nucleus.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Proenkephalin Antibody Summary

• Immunogen: Peptide with sequence C-RSHHQDGSDNEE corresponding to internal region according to NP_006202.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PENK
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 0.3 - 1 ug/ml
  • Immunohistochemistry-Frozen 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 37 kDa band observed in human adrenal gland lysates is reported in scientific literature (PMID: 9529003). IHC-Fr: Human hypothalamus shows staining of dense enkephalinergic axon plexus and scattered neuronal cell bodies (higher magnification inset) in the human infundibular nucleus.
• Control:

  Proenkephalin Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Proenkephalin Antibody

• enkephalin A
• preproenkephalin
• proenkephalin
• proenkephalin-A

Background

Enkephalins are small peptides derived from large precursers (pro-enkephalin A and B) containing multiple enkephalin copies. They are the most abundant opioid peptides in the body and are widely distributed in the brain and the peripheral nervous system and occur also in the adrenal medulla. Several types of neuroendocrine tumors, including pheochromocytomas, neuroblastomas and bronchial and gastrointestinal endocrine tumors, produce enkephalin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Proenkephalin Antibody (NBP1-06066)(1)

Publication using NBP1-06066

• Schiltz CA, Bremer QZ, Landry CF, Kelley AE. Food-associated cues alter forebrain functional connectivity as assessed with immediate early gene and proenkephalin expression. BMC Biol 2007-04-26 [PMID: 17462082]

Bioinformatics

• Gene Symbol: PENK
• Entrez:
  • 5179(Human)
• Uniprot:
  • P01210(Human)