![Western Blot: PRMT5 Antibody [NB100-56641] - Western blot analysis of PRMT5 in cell lysates from HeLa cells using NB100-56641 at 1:500. Twenty microgram of HeLa cell lysate was loaded per well of a mini gel.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Western-Blot-NB100-56641-img0001.jpg "Western Blot: PRMT5 Antibody [NB100-56641] - Western blot analysis of PRMT5 in cell lysates from HeLa cells using NB100-56641 at 1:500. Twenty microgram of HeLa cell lysate was loaded per well of a mini gel.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | This antibody was developed against a mixture of synthetic peptides corresponding to human PRMT5 amino acid sequence. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | PRMT5 |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS containing 0.1 mg/ml BSA. |
| Preservative | 0.02% Sodium Azide |
| Purity | Protein G purified |
| Publication using NB100-56641 | Applications | Species |
|---|---|---|
| Guo Zhigang, Zheng Li, Xu Hong et al. Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding. Nat Chem Biol. 2010-10-01 [PMID: 20729856] (Human) | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for PRMT5 Antibody (NB100-56641)Find related products by research area.
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![Western Blot: PRMT5 Antibody [NB100-56641] - analysis of PRMT5 in A) human HeLa and B) mouse NIH 3T3 lysate using this antibody.](http://images.novusbio.com/fullsize/PRMT5-Antibody-Western-Blot-NB100-56641-img0002.jpg "Western Blot: PRMT5 Antibody [NB100-56641] - analysis of PRMT5 in A) human HeLa and B) mouse NIH 3T3 lysate using this antibody.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")