PRMT5 Antibody

Images

 
Western Blot: PRMT5 Antibody [NB100-56641] - Western blot analysis of PRMT5 in cell lysates from HeLa cells using NB100-56641 at 1:500. Twenty microgram of HeLa cell lysate was loaded per well of a mini gel.
Western Blot: PRMT5 Antibody [NB100-56641] - analysis of PRMT5 in A) human HeLa and B) mouse NIH 3T3 lysate using this antibody.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

PRMT5 Antibody Summary

Immunogen
This antibody was developed against a mixture of synthetic peptides corresponding to human PRMT5 amino acid sequence.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PRMT5
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
Positive Control
PRMT5 Lysate (NBL1-14795)
HeLa Lysate (NB800-PC1)
Publications
Read Publication using NB100-56641.

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.1 mg/ml BSA.
Preservative
0.02% Sodium Azide
Purity
Protein G purified

Alternate Names for PRMT5 Antibody

  • EC 2.1.1.-
  • EC 2.1.1.125
  • Histone-arginine N-methyltransferase PRMT5
  • HMT1 hnRNP methyltransferase-like 5,72 kDa ICln-binding protein
  • HRMT1L5
  • IBP72
  • Jak-binding protein 1
  • JBP1
  • protein arginine methyltransferase 5
  • protein arginine N-methyltransferase 5
  • Shk1 kinase-binding protein 1 homolog
  • skb1 (S. pombe) homolog
  • SKB1 homolog (S. pombe)
  • SKB1
  • SKB1HsSKB1 homolog

Background

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT5, also known as Janus kinase-binding protein 1 (JBP1) and Skb1Hs (human homolog of Skb1 of fission yeast can catalyze the formation of omega-N(G)-monomethylarginine and symmetric omega-N(G), N(G')-dimethylarginine in a variety of proteins. Recombinant PRMT5 can mono and dimethylate histone 2A and myelin basic protein. PRMT5 appears to have lower specific enzyme activity than PRMT1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PRMT5 Antibody (NB100-56641)(1)

We have publications tested in 1 confirmed species: Human.


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Product General Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

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Bioinformatics

Gene Symbol PRMT5
Entrez
Uniprot