PRMT3 Antibody

Western Blot: PRMT3 Antibody [NBP2-26133] - NBP2-26133 (1ug/ml) staining of MCF7 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

PRMT3 Antibody Summary

• Description: Novus Biologicals Goat PRMT3 Antibody (NBP2-26133) is a polyclonal antibody validated for use in WB and ELISA. Anti-PRMT3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence DPKTLISEPCGIKH corresponding to internal region according to NP_005779.1, NP_001138638.1, NP_001138639.1.
• Specificity: This antibody is expected to recognize all reported isoforms (NP_005779.1; NP_001138638.1; NP_001138639.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PRMT3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in lysates of MCF7 is reported in scientific literature (PMID: 17439947).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for PRMT3 Antibody

• EC 2.1.1
• EC 2.1.1.-
• EC 2.1.1.319
• heterogeneous nuclear ribonucleoprotein methyltransferase-like 3
• Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
• HMT1 hnRNP methyltransferase-like 3 (S. cerevisiae)
• HMT1 hnRNP methyltransferase-like 3
• HRMT1L3
• PRMT3
• protein arginine methyltransferase 3
• protein arginine N-methyltransferase 3

Background

Type I protein arginine N-methyltransferases (PRMTs), such as PRMT3, catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins (Tang et al., 1998 [PubMed 9642256]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PRMT3 Antibody (NBP2-26133)(1)

Publication using NBP2-26133

• Kolbel K, Ihling C, Bellmann-Sickert K et al. Type I Arginine Methyltransferases PRMT1 and PRMT-3 Act Distributively. J Biol Chem 2009-03-27 [PMID: 19158082]

Bioinformatics

• Gene Symbol: PRMT3
• Entrez:
  • 10196(Human)