PRC1 Antibody (16F2)

Images

 
Immunocytochemistry/ Immunofluorescence: PRC1 Antibody (16F2) [NB100-74508] - Analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) shows staining in U251 Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and ...read more
Immunocytochemistry/ Immunofluorescence: PRC1 Antibody (16F2) [NB100-74508] - Analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) shows staining in A2058 Cells. PRC1 (green), F-Actin staining with Phalloidin (red) ...read more
Immunocytochemistry/ Immunofluorescence: PRC1 Antibody (16F2) [NB100-74508] - Analysis of PRC1 using PRC1 Monoclonal Antibody (16F2) shows staining in Hela Cells. PRC1 (green), F-Actin staining with Phalloidin (red) and ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IP
Clone
16F2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

PRC1 Antibody (16F2) Summary

Immunogen
Recombinant PRC1 protein.
Localization
Nuclear, depending on the phase of the cell cycle.
Specificity
PRC1 (16F2)
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PRC1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Immunocytochemistry/Immunofluorescence 1 - 2 ug/ml
  • Immunoprecipitation 1:10 - 1:500
Positive Control
PRC1 Lysate (NBL1-14729)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 1mg/ml BSA.
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for PRC1 Antibody (16F2)

  • anaphase spindle elongation 1 homolog
  • ASE1
  • MGC1671
  • MGC3669
  • protein regulating cytokinesis 1
  • protein regulator of cytokinesis 1

Background

Protein regulator of cytokinesis 1 (PRC1) is a spindle midzone-associated cyclin-dependent kinase substrate protein, responsible for microtubule binding and bundling. It is a mitotic, Cdk substrate necessary for cell cleavage and plays a role in the correct formation of the spindle midzone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for PRC1 Antibody (NB100-74508) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-74508AF405
Alexa Fluor 488 Labeled NB100-74508AF488
Alexa Fluor 647 Labeled NB100-74508AF647
Alexa Fluor 700 Labeled NB100-74508AF700
DyLight 350 Labeled NB100-74508UV
DyLight 405 Labeled NB100-74508V
DyLight 488 Labeled NB100-74508G
DyLight 680 Labeled NB100-74508FR
DyLight 755 Labeled NB100-74508IR

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Bioinformatics

Gene Symbol PRC1
Entrez
Uniprot