PRAS40 [p Thr246] Antibody (23GB3855)

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, Mycoplasma
• Clone: 23GB3855
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

PRAS40 [p Thr246] Antibody (23GB3855) Summary

• Immunogen: A synthesized peptide derived from human Phospho-PRAS40 (T246) (Uniprot: Q96B36)
• Modification: p Thr246
• Specificity: This antibody detects endogenous levels of AKT1S1 only when phosphorylated at T246
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AKT1S1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Knockdown Validated
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PRAS40 [p Thr246] Antibody (23GB3855)

• 40 kDa
• AKT1 substrate 1 (proline-rich)
• AKT1S1
• Lobe
• MGC2865
• PRAS40
• proline-rich AKT1 substrate 1,40 kDa proline-rich AKT substrate

Background

Proline Rich Akt Substrate (PRAS40) is a 40 kDa substrate of Akt. Akt and PRAS40 can be found in the phosphoinositide 3 kinase (PI3K) pathway, which plays a role in glucose uptake, cell growth, and apoptosis inhibition. PRAS40 is a 14-3-3 binding protein that reacts with insulin, but whose precise function is not yet known. It may bind SH3 and WW domain containing proteins resulting in a change of function. Activated Akt phosphorylates PRAS40 on threonine 246. Mutation of PRAS40 threonine 246 has been shown to be apoptotic.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AKT1S1