PRAK Antibody

Western Blot: PRAK Antibody [NBP2-19922] - Sample (30 ug of whole cell lysate) A: HepG2 10% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Po, Bv, Ch, Pm, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PRAK Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human PRAK. The exact sequence is proprietary.
• Localization: Cytoplasm (Probable); Nucleus
• Predicted Species: Mouse (98%), Rat (97%), Porcine (98%), Bovine (98%), Zebrafish (94%), Chicken (97%), Monkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPKAPK5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (100%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PRAK Antibody

• EC 2.7.11
• MAP kinase-activated protein kinase 5
• MAPK-activated protein kinase 5
• MAPKAP kinase 5
• MAPKAPK-5
• mitogen-activated protein kinase-activated protein kinase 5
• p38-regulated/activated protein kinase
• PRAKEC 2.7.11.1

Background

The protein encoded by this gene is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: MAPKAPK5
• Uniprot:
  • Q8IW41(Human)