PPP2R3A Antibody

Western Blot: PPP2R3A Antibody [NBP2-19908] - Sample (30 ug of whole cell lysate) A: NT2D1 B: U87-MG 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: PPP2R3A Antibody [NBP2-19908] - U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PPP2R3A protein stained by PPP2R3A antibody [C2C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.

Immunohistochemistry-Paraffin: PPP2R3A Antibody [NBP2-19908] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Western Blot: PPP2R3A Antibody [NBP2-19908] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PPP2R3A antibody [C2C3], C-term (NBP2-19908) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Western Blot: PPP2R3A Antibody [NBP2-19908] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with PPP2R3A antibody [C2C3], C-term (NBP2-19908) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

PPP2R3A Antibody Summary

• Description: Novus Biologicals Rabbit PPP2R3A Antibody (NBP2-19908) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human PPP2R3A. The exact sequence is proprietary.
• Predicted Species: Rhesus Macaque (97%), Bovine (92%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP2R3A
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 130 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for PPP2R3A Antibody

• PP2A subunit B isoform R3 isoform
• PP2A subunit B isoforms B72/B130
• PP2A subunit B isoforms B'-PR72/PR130
• protein phosphatase 2 (formerly 2A), regulatory subunit B' (PR 72), alphaisoform and (PR 130), beta isoform
• protein phosphatase 2 (formerly 2A), regulatory subunit B', alpha
• protein phosphatase 2, regulatory subunit B', alpha
• serine/threonine-protein phosphatase 2A regulatory subunit B' subunit alpha
• subunit B, R3 isoform

Background

Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the alpha subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: PPP2R3A
• Uniprot:
  • Q06190(Human)