PPP2CB Overexpression Lysate (Native)

Images

 
Western Blot: PPP2CB Overexpression Lysate (Adult Normal) [NBL1-14691] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPP2CB.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PPP2CB Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204491 and based on accession number NM_001009552. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform (PPP2CB), transcript variant 2, mRNA.
Gene
PPP2CB

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
35.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for PPP2CB

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PPP2CB Overexpression Lysate (Native)

  • EC 3.1.3.16
  • PP2Abeta
  • PP2A-beta
  • PP2CB
  • protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
  • protein phosphatase 2, catalytic subunit, beta isoform
  • protein phosphatase 2, catalytic subunit, beta isozyme
  • protein phosphatase 2A catalytic subunit, beta isoform
  • protein phosphatase type 2A catalytic subunit
  • serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform
  • serine/threonine-protein phosphatase 2A catalytic subunit beta isoform

Background

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-87232
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-31266
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
AF4339
Species: Hu, Mu, Rt
Applications: WB, IHC
MAB3000
Species: Hu, Mu, Rt
Applications: WB
NBP2-19907
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NB400-114
Species: Hu, Mu, Rt, Po, Ch, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB100-464
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P, PLA
NBP1-31348
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87931
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
1341-WF/CF
Species: Hu
NBP2-38600
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-384
Species: Hu, Mu, Rt, Ca
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
NB110-40619
Species: Hu, Mu
Applications: IP (-), WB, IHC-P
NBP2-15971
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB100-56637
Species: Hu, Mu
Applications: WB
NB100-247
Species: Hu
Applications: WB, IHC-P, IP, PLA
NBP1-83276
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBL1-14691
Species: Hu
Applications: WB

Publications for PPP2CB Lysate (NBL1-14691) (0)

There are no publications for PPP2CB Lysate (NBL1-14691).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for PPP2CB Lysate (NBL1-14691) (0)

There are no reviews for PPP2CB Lysate (NBL1-14691). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for PPP2CB Lysate (NBL1-14691) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional PPP2CB Products

Bioinformatics Tool for PPP2CB Lysate (NBL1-14691)

Discover related pathways, diseases and genes to PPP2CB Lysate (NBL1-14691). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for PPP2CB Lysate (NBL1-14691)

Discover more about diseases related to PPP2CB Lysate (NBL1-14691).
 

Pathways for PPP2CB Lysate (NBL1-14691)

View related products by pathway.

PTMs for PPP2CB Lysate (NBL1-14691)

Learn more about PTMs related to PPP2CB Lysate (NBL1-14691).
 

Research Areas for PPP2CB Lysate (NBL1-14691)

Find related products by research area.

Blogs on PPP2CB

There are no specific blogs for PPP2CB, but you can read our latest blog posts.

Customers Who Bought This Also Bought

IL-4 [Unconjugated]
204-IL-010/CF
PPP2CB Antibody
NBP1-32069

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our PPP2CB Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol PPP2CB