PPIH Overexpression Lysate

Images

 
Western Blot: PPIH Overexpression Lysate (Adult Normal) [NBL1-14650] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPIH.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PPIH Overexpression Lysate Summary

Description

PPIH Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC201529

Accession#: NM_006347

Protein Tag: C-MYC/DDK
Gene
PPIH

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PPIH Overexpression Lysate

  • cyclophilin H
  • CYP20
  • CypH
  • CYPHpeptidyl prolyl isomerase H (cyclophilin H)
  • EC 5.2.1.8
  • MGC5016
  • peptidyl-prolyl cis-trans isomerase H
  • peptidylprolyl isomerase H (cyclophilin H)
  • PPIase H
  • Rotamase H
  • Small nuclear ribonucleoprotein particle-specific cyclophilin H
  • SnuCyp-20
  • USA-CyP SnuCyp-20
  • USA-CYPCYP-20
  • U-snRNP-associated cyclophilin SnuCyp-20
  • U-snRNP-associated cyclophilin SunCyp-20

Background

The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol PPIH