PPHLN1 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: PPHLN1 Antibody [NBP1-89480] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.

Staining of human cerebral cortex, colon, liver and testis using Anti-PPHLN1 antibody NBP1-89480 (A) shows similar protein distribution across tissues to independent antibody NBP2-38431 (B).

Analysis in human testis and pancreas tissues using NBP1-89480 antibody. Corresponding PPHLN1 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human pancreas shows negative to very weak positivity in exocrine glandular cells.

Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons.

Immunohistochemistry-Paraffin: PPHLN1 Antibody [NBP1-89480] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.

Staining of human liver shows weak nuclear positivity in hepatocytes.

Western Blot: PPHLN1 Antibody [NBP1-89480] - Human foreskin fibroblasts whole cell lysates. Antibody at 1:1000. Image submitted by a verified customer review.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PPHLN1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PPHLN1 Antibody - BSA Free (NBP1-89480) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-PPHLN1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KKPPLLDRPGEGSYNRYYSHVDYRDYDEGRSFSHDRRSGPPHRGDESGYRWTRDDHSASRQPEYRDMRDGFRRKSFYSSHYARERSPYKRDNTFFRESPVGRKDSPHSR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPHLN1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. PPHLN1 antibody validated for WB from a verified customer review.
• Control Peptide:

  PPHLN1 Protein (NBP1-89480PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for PPHLN1 Antibody - BSA Free

• Gastric cancer antigen Ga50
• HSPC232
• MGC48786
• periphilin 1
• periphilin-1

Background

PPHLN1 is encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PPHLN1 Antibody (NBP1-89480)(1)

Publication using NBP1-89480

• Tchasovnikarova IA, Timms RT, Matheson NJ et al. GENE SILENCING. Epigenetic silencing by the HUSH complex mediates position-effect variegation in human cells. Science 2015 Jun 26 [PMID: 26022416] (WB, ICC/IF)

Review for PPHLN1 Antibody (NBP1-89480) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 05/14/2019

Summary

• Application: Western Blot
• Sample Tested: Human Foreskin Fibroblasts
• Species: Human
• Lot: D118905

Bioinformatics

• Gene Symbol: PPHLN1