PPAP2A Overexpression Lysate

Images

 
Western Blot: PPAP2A Overexpression Lysate (Adult Normal) [NBL1-14625] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PPAP2A.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

PPAP2A Overexpression Lysate Summary

Description

PPAP2A Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC208064

Accession#: NM_003711

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
PPAP2A

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for PPAP2A Overexpression Lysate

  • EC 3.1.3.4
  • lipid phosphate phosphohydrolase 1
  • lipid phosphate phosphohydrolase 1a
  • LLP1a
  • LPP1PAP2a2
  • PAP2
  • PAP2a
  • PAP2-alpha
  • PAP-2aPAP2alpha2
  • PAPalpha1
  • Phosphatidate phosphohydrolase type 2a
  • Phosphatidic acid phosphatase 2a
  • phosphatidic acid phosphatase type 2A
  • phosphatidic acid phosphohydrolase type 2a
  • type 2 phosphatidic acid phosphohydrolase
  • type-2 phosphatidic acid phosphatase alpha

Background

The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is an integral membrane glycoprotein, and has been shown to be a surface enzyme that plays an active role in the hydrolysis and uptake of lipids from extracellular space. The expression of this gene is found to be regulated by androgen in a prostatic adenocarcinoma cell line. At least two alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (1), also named PAP2-a1, contains an in-frame alternate exon within the coding region, when compared to variant 2. The resulting isoform (1) has a distinct internal segment, and is 1 aa shorter, as compared to isoform 2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Publications for PPAP2A Lysate (NBL1-14625) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol PPAP2A