PPAN Antibody - BSA Free

Immunoprecipitation:PPAN AntibodyNBP3-29745] -Detection of human PPAN by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PPAN antibody NBP3-29745 (lot NBP3-29745-1) used for IP at 6 µg per reaction. PPAN was also immunoprecipitated by rabbit anti-PPAN antibody . For blotting immunoprecipitated PPAN, NBP3-29745 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

PPAN Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit PPAN Antibody - BSA Free (NBP3-29745) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Between 375 and 425
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPAN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-citrate/phosphate buffer, pH 7 to 8
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for PPAN Antibody - BSA Free

• Brix domain-containing protein 3
• homolog of S. cerevisiae SSF1
• MGC14226
• MGC45852
• peter pan (Drosophila) homolog
• peter pan homolog (Drosophila)
• Peter Pan homolog
• second-step splicing factor 1
• SSF
• Ssf-1
• SSF1BXDC3SSF-1
• SSF2
• suppressor of sterile four 1
• suppressor of SWI4 1 homolog

Background

PPAN is encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. This gene was found to cotranscript with P2RY11/P2Y(11), an immediate downstream gene on the chromosome that encodes a ATP receptor. The chimeric transcripts of this gene and P2RY11 were found to be ubiquitously present and regulated during granulocytic differentiation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPAN