PP2C gamma/PPM1G Antibody (S05-8C4)

Western Blot: PP2C gamma/PPM1G Antibody (S05-8C4) [NBP3-19743] - Western blot detection of PP2C gamma/PPM1G in K562 (Lane 1), Rat Brain (Lane 2), C6 (Lane 3), 3T3 (Lane 4), Hela (Lane 5) cell lysates using NBP3-19743 (1:1000 diluted). Predicted band size: 59kDa. Observed band size: 75kDa.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: S05-8C4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

PP2C gamma/PPM1G Antibody (S05-8C4) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of human PP2C gamma/PPM1G (Uniprot # O15355)
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: PPM1G
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/2000-1/10000
• Theoretical MW: 75 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for PP2C gamma/PPM1G Antibody (S05-8C4)

• EC 3.1.3.16
• MGC1675
• MGC2870
• PP2C gamma
• PP2C, gamma
• PP2CG
• PP2CGAMMA
• PP2C-gamma
• PPM1C
• PPM1G
• PPP2CG
• Protein phosphatase 1C
• protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
• protein phosphatase 1G
• protein phosphatase 2, catalytic subunit, gamma isoform
• protein phosphatase 2C gamma isoform
• Protein phosphatase 2C isoform gamma
• Protein phosphatase magnesium-dependent 1 gamma
• protein phosphatase, Mg2+/Mn2+ dependent, 1G

Background

PPM1G is encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Studies of a similar gene in mice suggested a role of this phosphatase in regulating cell cycle progression. Alternatively spliced transcript variants encoding the same protein have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPM1G