PP2C alpha/PPM1A Antibody (4E11) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: PP2C alpha/PPM1A Antibody (4E11) [H00005494-M03] - Analysis of monoclonal antibody to PPM1A on HeLa cell. Antibody concentration 10 ug/ml

Immunohistochemistry-Paraffin: PP2C alpha/PPM1A Antibody (4E11) [H00005494-M03] - Analysis of monoclonal antibody to PPM1A on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml

Proximity Ligation Assay: PP2C alpha/PPM1A Antibody (4E11) [H00005494-M03] - Analysis of protein-protein interactions between AKT1 and PPM1A. HeLa cells were stained with anti-AKT1 rabbit purified polyclonal 1:1200 and anti-PPM1A mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction c

Sandwich ELISA: PP2C alpha/PPM1A Antibody (4E11) [H00005494-M03] - Detection limit for recombinant GST tagged PPM1A is 3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, Func, ICC/IF, IHC
• Clone: 4E11
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

PP2C alpha/PPM1A Antibody (4E11) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: PPM1A (AAH26691, 1 a.a. ~ 382 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGAFLDKPKMEKHNAQGQGNGLRYGLSSMQGWRVEMEDAHTAVIGLPSGLESWSFFAVYDGHAGSQVAKYCCEHLLDHITNNQDFKGSAGAPSVENVKNGIRTGFLEIDEHMRVMSEKKHGADRSGSTAVGVLISPQHTYFINCGDSRGLLCRNRKVHFFTQDHKPSNPLEKERIQNAGGSVMIQRVNGSLAVSRALGDFDYKCVHGKGPTEQLVSPEPEVHDIERSEEDDQFIILACDGIWDVMGNEELCDFVRSRLEVTDDLEKVCNEVVDTCLYKGSRDNMSVILICFPNAPKVSPEAVKKEAELDKYLECRVEEIIKKQGEGVPDLVHVMRTLASENIPSLPPGGELASKRNVIEAVYNRLNPYKNDDTDSTSTDDMW
• Specificity: PPM1A - protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform (4E11)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPM1A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Proximity Ligation Assay
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for PP2C alpha/PPM1A Antibody (4E11) - Azide and BSA Free

• EC 3.1.3.16
• FLJ42306
• MGC9201
• MMPa-2
• MPPa-1
• phosphatase 2C alpha
• PP2C alpha
• Pp2c1
• PP2CA
• PP2Calpha
• PP2C-ALPHA
• PPM1A
• PPPM1A
• protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
• protein phosphatase 1A
• protein phosphatase 2C alpha isoform
• Protein phosphatase 2C isoform alpha
• Protein phosphatase IA
• protein phosphatase, Mg2+/Mn2+ dependent, 1A

Background

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for PP2C alpha/PPM1A Antibody (H00005494-M03)(1)

Publication using H00005494-M03

• Jung-Sun L, Eun-Ju L, Jae-Hyun L et al. Autoantibodies against Protein Phosphatase Magnesium-Dependent 1A as a Biomarker for Predicting Radiographic Progression in Ankylosing Spondylitis Treated with Anti-Tumor Necrosis Factor Agents. J Clin Med. 2020-12-07 [PMID: 33297507]

Bioinformatics

• Gene Symbol: PPM1A
• Entrez:
  • 5494(Human)
• Uniprot:
  • AAH26691(Human)