PP1 alpha/PPP1A Antibody (4G3)

Images

 
Western Blot: PP1 alpha/PPP1A Antibody (4G3) [NBP1-04334] - Cell lysates of HepG2 (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1CA (1:1000). Proteins were visualized using a ...read more
Immunocytochemistry/ Immunofluorescence: PP1 alpha/PPP1A Antibody (4G3) [NBP1-04334] - Analysis of PPP1CA in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPP1CA antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
Clone
4G3
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

PP1 alpha/PPP1A Antibody (4G3) Summary

Immunogen
Recombinant human PPP1CA (30-299aa) purified from E. coli
Specificity
Anti-human PPP1CA mAb, clone 4G3, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PPP1CA protein.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
PPP1CA
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:100

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for PP1 alpha/PPP1A Antibody (4G3)

  • EC 3.1.3.16
  • PP1 alpha
  • PP1alpha
  • PP-1AMGC15877
  • PPP1A
  • PPP1AMGC1674
  • PPP1CA
  • protein phosphatase 1, catalytic subunit, alpha isoform
  • protein phosphatase 1, catalytic subunit, alpha isozyme
  • serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit
  • serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Background

Protein phosphatase 1, catalytic subunit, alpha isoform, also known as PPP1CA, is one of the three catalytic subunits of protein phosphatase 1. The protein is highly conserved in eukaryotic cells and play a role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase 2. It is participate in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis and apoptosis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol PPP1CA
Entrez
Uniprot