PP1 alpha/PPP1A Antibody (4G3) - BSA Free

Western Blot: PP1 alpha/PPP1A Antibody (4G3) [NBP1-04334] - Cell lysates of HepG2 (45ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1CA (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Immunocytochemistry/Immunofluorescence: PP1 alpha/PPP1A Antibody (4G3) [NBP1-04334] - Analysis of PPP1CA in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human PPP1CA antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 4G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

PP1 alpha/PPP1A Antibody (4G3) - BSA Free Summary

• Description: Novus Biologicals Mouse PP1 alpha/PPP1A Antibody (4G3) - BSA Free (NBP1-04334) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human PP1 alpha/PPP1A (30-299aa) purified from E. coli
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PPP1CA
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 10% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for PP1 alpha/PPP1A Antibody (4G3) - BSA Free

• EC 3.1.3.16
• PP1 alpha
• PP1alpha
• PP-1AMGC15877
• PPP1A
• PPP1AMGC1674
• PPP1CA
• protein phosphatase 1, catalytic subunit, alpha isoform
• protein phosphatase 1, catalytic subunit, alpha isozyme
• serine/threonine protein phosphatase PP1-alpha 1 catalytic subunit
• serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Background

Protein phosphatase 1, catalytic subunit, alpha isoform, also known as PPP1CA, is one of the three catalytic subunits of protein phosphatase 1. The protein is highly conserved in eukaryotic cells and play a role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase 2. It is participate in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis and apoptosis

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1CA
• Uniprot:
  • NP_002699(Human)