Potassium Channel Kv2.2 Antibody - Azide Free

Western Blot: Potassium Channel Kv2.2 Antibody [NB300-261] - Rat Brain - The immunolabeling is blocked by preadsorption with the peptide used as antigen (Peptide block).

• Reactivity: Rt, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

Potassium Channel Kv2.2 Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit Potassium Channel Kv2.2 Antibody - Azide Free (NB300-261) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide corresponding to amino acid residues specific to the Kv2.2 subunit conjugated to KLH. Accession # Q63099
• Localization: Cell Membrane
• Specificity: Specific for endogenous levels of the ~125 kDa voltage gated potassium channel, Kv2.2 subunit.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: KCNB2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000
  • Immunohistochemistry-Frozen 1:10-1:500
  • Western Blot 1:1000
• Theoretical MW: 125 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for Potassium Channel Kv2.2 Antibody - Azide Free

• delayed rectifier potassium channel protein
• KCNB2
• KV2.2
• Potassium Channel Kv2.2
• potassium voltage-gated channel subfamily B member 2
• potassium voltage-gated channel, Shab-related subfamily, member 2
• voltage-gated potassium channel subunit Kv2.2

Background

Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pongs, 1999). Moreover differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK)channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety on non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes (Hwang et al., 1993; Yan et al., 2004; Michaelevski et al., 2003). Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells (Blaine and Ribera, 1998; Burger and Ribera, 1996).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KCNB2
• Entrez:
  • 117105(Rat)
• Uniprot:
  • Q63099(Rat)