PON3 Antibody (454929) [Alexa Fluor® 532]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 454929
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 532

PON3 Antibody (454929) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant human PON3
  Ala30-Leu354
  Accession # AAH70374
• Specificity: Detects endogenous human, mouse, and rat PON3 in Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) PON1 or rhPON2.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for PON3 Antibody (454929) [Alexa Fluor® 532]

• EC 3.1.1.2
• EC 3.1.1.81
• EC 3.1.8.1
• paraoxanase-3
• paraoxonase 3
• PON3
• serum paraoxonase/lactonase 3

Background

The paraoxonase (PON) gene family of antioxidant enzymes includes three known members located adjacent to each other on chromosome 7. Paraoxonase/lactonase 3 (PON3) is a 354 amino acid, 39.6 kDa protein that is secreted into the bloodstream and associates with high-density lipoprotein (HDL). PON3 has lactonase activity (EC 3.1.1) and rapidly hydrolyzes lactones. PON3 exhibits no paraoxonase and very limited arylesterase activities. PON3 is an important antioxidant and can inhibit the oxidation of low-density lipoprotein (LDL), a function that may slow the initiation and progression of atherosclerosis. PON3 is glycosylated at Asn323 and alternatively spliced variants encoding different protein isoforms have been described; however, only one has been fully characterized. Human PON3 shares 81% amino acid identity with mouse and rat PON3.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.