Polycystin-1/PKD1 Antibody

Immunohistochemistry-Paraffin: Polycystin-1/PKD1 Antibody [NBP1-52552] - Analysis of anti-PKD1 antibody with human kidney at concentration 3.75 ug/ml.

Immunohistochemistry-Paraffin: Polycystin-1/PKD1 Antibody [NBP1-52552] - Analysis of anti-PKD1 antibody with human spleen at concentration 3.75 ug/ml.

• Reactivity: Hu, Pm
• Applications: ELISA, IHC, IP, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Polycystin-1/PKD1 Antibody Summary

• Immunogen: Synthetic peptide C-RTPLRAKNKVHP from the C-terminus of human Polycystin-1/PKD1 (NP_001009944.1; NP_000287.2).
• Epitope: C-RTPLRAKNKVHP from the C-Terminus
• Specificity: Human PKD1. This antibody is expected to recognize both reported isoforms (NP_001009944.1 and NP_000287.2).
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PKD1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Immunoprecipitation
  • Peptide ELISA
• Application Notes: .

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (83%), Feline (83%), Canine (83%), Equine (83%).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: TBS (pH 7.3) and 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Polycystin-1/PKD1 Antibody

• Autosomal dominant polycystic kidney disease 1 protein
• PBP
• Pc-1
• PKD1
• polycystic kidney disease 1 (autosomal dominant)
• polycystic kidney disease-associated protein
• polycystin 1
• Polycystin1
• Polycystin-1
• transient receptor potential cation channel, subfamily P, member 1
• TRPP1

Background

Polycystin-1 (also PKD1) is a 500-550 kDa member of the polycystin family of proteins. It is expressed in renal tubule primary cilia, and the membrane region that forms adherens junctions. Polycystin-1 binds to polycystin-2, promoting its insertion into the cell membrane, and regulating its calcium channel activity. In conjunction with polycystin-2, it detects fluid flow and converts this information into calcium signals. It also exists in the ER, where it negatively modulates polycystin-2 mediated calcium release. Mature human polycystin-1 is a 4280 amino acid (aa), 11 transmembrane glycoprotein. Its N-terminal extracellular region (aa 24-3074) is highly modular, and contains a C-type lecin domain, multiple Leu-rich repeats and PKD domains, one GPS region and a PLAT, REJ, WSC and LDLR domain. The cytoplasmic C-terminus (aa 4107-4303) contains a polycystin-2 coiled-coil interaction domain (aa 4220-4251). A percentage of polycystin-1 undergoes autoproteolysis after Leu3048, generating a presumed N-terminal fragment of ~ 350-400 kDa, and a membrane-bound C-terminal fragment of 150 kDa. Over aa 4141-4303, human polycystin-1 shares 71% aa identity with mouse polycystin-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PKD1
• Entrez:
  • 5310(Human)
• Uniprot:
  • P98161(Human)