POLR3K Antibody (3F5) - Azide and BSA Free

Western Blot: POLR3K Antibody (3F5) [H00051728-M01] - Analysis of POLR3K expression in transfected 293T cell line by POLR3K monoclonal antibody (M01), clone 3F5.Lane 1: POLR3K transfected lysate(12.3 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: POLR3K Antibody (3F5) [H00051728-M01] - Analysis of monoclonal antibody to POLR3K on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: POLR3K Antibody (3F5) [H00051728-M01] - Detection limit for recombinant GST tagged POLR3K is approximately 0.1ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 3F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

POLR3K Antibody (3F5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse POLR3K Antibody (3F5) - Azide and BSA Free (H00051728-M01) is a monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: POLR3K (AAH11932, 1 a.a. ~ 108 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLLFCPGCGNGLIVEEGQRCHRFACNTCPYVHNITRKVTNRKYPKLKEVDDVLGGAAAWENVDSTAESCPKCEHPRAYFMQLQTRSADEPMTTFYKCCNAQCGHRWRD
• Specificity: POLR3K - polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLR3K
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against recombinant protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for POLR3K Antibody (3F5) - Azide and BSA Free

• C11
• DNA-directed RNA polymerase III subunit K
• DNA-directed RNA polymerase III subunit RPC10
• DNA-directed RNA polymerases III 12.5 kDa polypeptide
• hRPC11
• HsC11p
• polymerase (RNA) III (DNA directed) polypeptide K (12.3 kDa)
• polymerase (RNA) III (DNA directed) polypeptide K, 12.3 kDa
• RNA polymerase III 12.5 kDa subunit
• RNA polymerase III subunit (hRPC11)
• RNA polymerase III subunit C10
• RNA polymerase III subunit C11
• RNA polymerase III subunit CII
• RPC10
• RPC11C11-RNP3
• RPC12.5

Background

This gene encodes a small essential subunit of RNA polymerase III, the polymerase responsible for synthesizing transfer and small ribosomal RNAs in eukaryotes. The carboxy-terminal domain of this subunit shares a high degree of sequence similarity to the carboxy-terminal domain of an RNA polymerase II elongation factor. This similarity in sequence is supported by functional studies showing that this subunit is required for proper pausing and termination during transcription.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR3K
• Entrez:
  • 51728(Human)
• OMIM:
  • 606007(Human)
• Uniprot:
  • AAH11932(Human)