POLR3D Recombinant Protein Antigen

• Reactivity: Hu
• Applications: AC

POLR3D Recombinant Protein Antigen Summary

• Description: A recombinant protein antigen with a N-terminal His6-ABP tag corresponding to human POLR3D.

  Source: E. coli

  Amino Acid Sequence: PRPLLTGARGLIGRRPAPPLTPGRLPSIRSRDLTLGGVKKKTFTPNIISRKIKEEPK

  Fusion Tag: N-terminal His6ABP (ABP = Albumin Binding Protein derived from Streptococcal Protein G)

  This product is intended to be used as a blocking antigen for antibody competition assays. Any other use of this antigen is done at the risk of the user. The use of this product for commercial production is strictly prohibited. Please contact technical support if you have any questions.

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein Antigen
• Gene: POLR3D
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • Antibody Competition 10 - 100 molar excess
• Application Notes: This recombinant antigen is only intended to be used as a blocking agent to confirm antibody specificity with the corresponding antibody, catalog number NBP2-55887.

  It is purified by IMAC chromatography, and the expected concentration is greater than 0.5 mg/ml.

  For current lot information, including availability, please contact our technical support team click nb-technical@bio-techne.com

• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS and 1M Urea, pH 7.4.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Alternate Names for POLR3D Recombinant Protein Antigen

• BN51 (BHK21) temperature sensitivity complementing
• BN51
• BN51TDNA-directed RNA polymerase III 47 kDa polypeptide
• DNA-directed RNA polymerase III subunit D
• DNA-directed RNA polymerase III subunit RPC4
• polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
• Protein BN51
• RNA polymerase III 47 kDa subunit
• RNA polymerase III subunit C4
• RPC4
• RPC53 homolog
• temperature sensitive complementation, cell cycle specific, tsBN51
• TSBN51RPC53

Background

RNA polymerase III subunit D (POLR3D) is one of 17 subunits of the RNA polymerase III (PolIII) which synthesizes small RNAs, such as 5S rRNA and tRNA. POLR3D include RNA polymerase III subunit C4, RPC4, DNA-directed RNA polymerase III subunit D, DNA-directed RNA polymerase III 47 kDa polypeptide, RPC53, protein BN51, TSBN51, and BN51T.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: POLR3D