POLR2F Antibody (2G2) - Azide and BSA Free

Western Blot: POLR2F Antibody (2G2) [H00005435-M02] - Analysis of POLR2F expression in transfected 293T cell line by POLR2F monoclonal antibody (M02), clone 2G2.Lane 1: POLR2F transfected lysate (Predicted MW: 14.5 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: POLR2F Antibody (2G2) [H00005435-M02] - Analysis of monoclonal antibody to POLR2F on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: POLR2F Antibody (2G2) [H00005435-M02] - Detection limit for recombinant GST tagged POLR2F is 0.3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

POLR2F Antibody (2G2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: POLR2F (NP_068809.1, 1 a.a. ~ 127 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSDNEDNFDGDDFDDVEEDEGLDDLENAEEEGQENVEILPSGERPQANQKRITTPYMTKYERARVLGTRALQIAMCAPVMVELEGETDPLLIAMKELKARKIPIIIRRYLPDGSYEDWGVDELIITD
• Specificity: This product is specific for Human POLR2F monoclonal antibody (M02), clone 2G2 [Gene ID: 5435].
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: POLR2F
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for POLR2F Antibody (2G2) - Azide and BSA Free

• DNA-directed RNA polymerase II subunit F
• DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide
• HRBP14.4
• II, and III subunit RPABC2
• polymerase (RNA) II (DNA directed) polypeptide F
• RNA Polymerase II subunit 14.4 kD
• RNA polymerases I, II, and III subunit ABC2
• RPB6 homolog
• RPB6

Background

POLR2F encodes the sixth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes, that is also shared by the other two DNA-directed RNA polymerases. In yeast, this polymerase subunit, in combination with at least two other subunits, forms a structure that stabilizes the transcribing polymerase on the DNA template. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: POLR2F
• Uniprot:
  • NP_068809.1(Human)