Podocan Antibody

Podocan was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse Podocan Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3104) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to vascular endothelial cells within glomeruli. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Podocan Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Podocan
  Val24-Arg611
  Accession # Q7TQ62
• Specificity: Detects mouse Podocan in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human Podocan is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PODN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podocan Antibody

• PCAN
• PODN
• podocan proteoglycan
• Podocan
• SLRR5A
• SLRR5AMGC24995

Background

Mouse Podocan is a 95 kDa, secreted glycoprotein that is a class V member of the small leucine rich protein gene family (1, 2). It is synthesized as a 611 amino acid (aa) precursor that contains a 23 aa signal sequence, a cysteine-rich region, a series of leucine rich repeats (LRRs), and an extensive acidic C-terminal domain (4, 5). The 15 aa cysteine-rich region (aa 69-84) shows a CX3CXCX7C motif, qualifying it as a class V SLRP family member. This is followed by twenty LRRs, thirteen of which are type T (4xLeu; 1xPhe) and seven type S (4xLeu; 2xPro). The LRRs run uninterrupted from aa 89-559. The C-terminal seventeen amino acids contain fourteen Glu residues. The negative charge associated with these residues may play a role in basement membrane permeability (4). Mature mouse Podocan is 93% and 94% aa identical to human and canine Podocan, respectively. Over the last 504 aa, mouse Podocan shares 98% aa sequence identity with rat Podocan. Podocan is apparently secreted by podocytes and vascular endothelial cells, and deposited in the underlying basement membrane (4). Podocan is known to bind to type I collagen, and have an inhibitory effect on the migration of Podocan-transfected CHO cells (5). The significance of this is unclear.

1. Matsushima, N. et al. (2000) Proteins 38:210.
2. Matsushima, N. et al. (2004) Proteins 54:394.
3. Ross, M.D. et al. (2005) Cell. Mol. Life Sci. 62:2771.
4. Ross, M.D. et al. (2003) J. Biol. Chem. 278:33248.
5. Shimizu-Hirota, R. et al. (2004) FEBS Lett. 563:69.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PODN
• Uniprot:
  • Q7TQ62()