Podocan Antibody [Alexa Fluor® 594]

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 594

Podocan Antibody [Alexa Fluor® 594] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Podocan
  Val24-Arg611
  Accession # Q7TQ62
• Specificity: Detects mouse Podocan in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant human Podocan is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Podocan Antibody [Alexa Fluor® 594]

• PCAN
• PODN
• podocan proteoglycan
• Podocan
• SLRR5A
• SLRR5AMGC24995

Background

Mouse Podocan is a 95 kDa, secreted glycoprotein that is a class V member of the small leucine rich protein gene family (1, 2). It is synthesized as a 611 amino acid (aa) precursor that contains a 23 aa signal sequence, a cysteine-rich region, a series of leucine rich repeats (LRRs), and an extensive acidic C-terminal domain (4, 5). The 15 aa cysteine-rich region (aa 69-84) shows a CX3CXCX7C motif, qualifying it as a class V SLRP family member. This is followed by twenty LRRs, thirteen of which are type T (4xLeu; 1xPhe) and seven type S (4xLeu; 2xPro). The LRRs run uninterrupted from aa 89-559. The C-terminal seventeen amino acids contain fourteen Glu residues. The negative charge associated with these residues may play a role in basement membrane permeability (4). Mature mouse Podocan is 93% and 94% aa identical to human and canine Podocan, respectively. Over the last 504 aa, mouse Podocan shares 98% aa sequence identity with rat Podocan. Podocan is apparently secreted by podocytes and vascular endothelial cells, and deposited in the underlying basement membrane (4). Podocan is known to bind to type I collagen, and have an inhibitory effect on the migration of Podocan-transfected CHO cells (5). The significance of this is unclear.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.