Recombinant Human PNMT Protein

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SDS-Page: PNMT Protein [NBP1-30280] - PNMT, 30.8 Da (282aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

Order Details

Recombinant Human PNMT Protein Summary

Description
An un-tagged recombinant protein corresponding to the amino acids 1-282 of Human PNMT

Source: E.coli

Amino Acid Sequence: MSGADRSPNA GAAPDSAPGQ AAVASAYQRF EPRAYLRNNY APPRGDLCNP NGVGPWKLRC LAQTFATGEV SGRTLIDIGS GPTVYQLLSA CSHFEDITMT DFLEVNRQEL GRWLQEEPGA FNWSMYSQHA CLIEGKGECW QDKERQLRAR VKRVLPIDVH QPQPLGAGSP APLPADALVS AFCLEAVSPD LASFQRALDH ITTLLRPGGH LLLIGALEES WYLAGEARLT VVPVSEEEVR EALVRSGYKV RDLRTYIMPA HLQTGVDDVK GVFFAWAQKV GL

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
PNMT
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
30.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human PNMT Protein

  • EC 2.1.1
  • EC 2.1.1.28
  • Noradrenaline N-methyltransferase
  • PENT
  • PENTMGC34570
  • phenylethanolamine N-methylase
  • phenylethanolamine N-methyltransferase
  • PNMT
  • PNMTase

Background

PNMT, also known as phenylethanolamine N-methyltransferase, is an enzyme found in the adrenal medulla that catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Recombinant PNMT protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol PNMT
Entrez