PNMT Antibody [Alexa Fluor® 647]

• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 647

PNMT Antibody [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human PNMT
  Ser2-Leu282
  Accession # P11086
• Specificity: Detects human PNMT in direct ELISAs and Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human (rh) INMT and rhNNMT is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

PNMT (Phenylethanolamine N-MethylTransferase; also PENT and Noradrenaline N-methyltransferase) is a monomeric, 30-34 kDa cytoplasmic member of the NNMT/PNMT/TEMT family of enzymes. It has limited expression, being found in Leydig, skeletal muscle and B cells, adrenal chromaffin cells, select adrenergic neurons of the vagus nerve, periventricular grey and reticular formation, and specialized adrenalin-containing cells contained within the heart. PNMT converts noradrenalin into adrenalin by transferring a methyl group from AdoMet to the terminal amine group of noradrenalin. Human PNMT is 282 amino acids (aa) in length and contains an active enzyme site between aa 14-279. Full length human PNMT1 shares 81% aa sequence identity with mouse PNMT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.