PMP70 Antibody

Images

 
Western Blot: PMP70 Antibody [NBP1-20871] - (0.2ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: PMP70 Antibody [NBP1-20871] - 3.8ug/ml staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

PMP70 Antibody Summary

Immunogen
Peptide with sequence C-PDGREDQKRKGISD corresponding to internal region according to NP_002849.1.
Epitope
C terminus
Marker
Proxisomal Membrane Marker
Specificity
This antibody is expected to recognize one isoform (NP_002849.1) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ABCD3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 - 0.6 ug/ml
  • Immunohistochemistry 2.5 - 3.8 ug/ml
  • Immunohistochemistry-Paraffin 2.5 - 3.8 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 70 kDa band observed in human kidney lysates (calculated MW of 75.5 kDa band according to NP_002849.1). IHC-P: Human testis shows vesicular staining in Sertoli cells.
Publications
Read Publications using
NBP1-20871 in the following applications:

  • WB
    1 publication

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for PMP70 Antibody

  • 70 kDa peroxisomal membrane protein
  • ABC43
  • ATP-binding cassette, sub-family D (ALD), member 3
  • peroxisomal membrane protein 1 (70kD, Zellweger syndrome)
  • Peroxisomal membrane protein-1 (70kD)
  • PMP70ATP-binding cassette sub-family D member 3
  • PXMP1dJ824O18.1 (ATP-binding cassette, sub-family D (ALD), member 3 (PMP70, PXMP1))
  • ZWS2

Background

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ALD subfamily, which is involved in peroxisomal import of fatty acids and/or fatty acyl-CoAs in the organelle. All known peroxisomal ABC transporters are half transporters which require a partner half transporter molecule to form a functional homodimeric or heterodimeric transporter. This peroxisomal membrane protein likely plays an important role in peroxisome biogenesis. Mutations have been associated with some forms of Zellweger syndrome, a heterogeneous group of peroxisome assembly disorders. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for PMP70 Antibody (NBP1-20871)(2)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


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WB
(1)
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(1)
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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol ABCD3
Entrez
Uniprot